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Literature summary for 7.1.1.8 extracted from
- Electrostatic attraction between cytochrome bc1 and cytochrome c affects kinetics of cytochrome c reduction (2008), Biochemistry (Moscow), 73, 870-880.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Paracoccus denitrificans | Paracoccus denitrificans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a functional cyt bc1 complex with a truncated cyt c1: the coding region of the fbcC gene between triplet 39 and 202, specifying the complete highly acidic domain of the Paracoccus denitrificans cyt c1, is deleted in frame (removing nucleotides 2279 to 2766) | Paracoccus denitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
2 ferricytochrome c | wild-type protein | Paracoccus denitrificans | |
| 0.00714 | - |
2 ferricytochrome c | mutant with truncated cytochrome c1 | Paracoccus denitrificans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
ATCC 13543 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| differential centrifugation | Paracoccus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.29 | - |
mutant with truncated cytochrome c1, membrane fraction | Paracoccus denitrificans |
| 1.35 | - |
wild type, membrane fraction | Paracoccus denitrificans |
| 308 | - |
mutant with truncated cytochrome c1, purified bc1 complex | Paracoccus denitrificans |
| 469 | - |
wild type, purified bc1 complex | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol + 2 ferricytochrome c | - |
Paracoccus denitrificans | 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone + ferrocytochrome c + H+ [side 2] | - |
? |  |
| 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol + 2 ferricytochrome c | - |
Paracoccus denitrificans | 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone + 2 ferrocytochrome c + H+ [side 2] | - |
? | |
| coenzyme Q1H2 + 2 ferricytochrome c | - |
Paracoccus denitrificans | coenzyme Q1 + 2 ferrocytochrome c + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 3 catalytic subunits | Paracoccus denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ubiquinol-cytochrome c reductase | - |
Paracoccus denitrificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 165 | - |
2 ferricytochrome c | mutant with truncated cytochrome c1, membrane fraction | Paracoccus denitrificans | |
| 170 | - |
2 ferricytochrome c | wild type, membrane fraction | Paracoccus denitrificans | |
| 517 | - |
2 ferricytochrome c | mutant with truncated cytochrome c1, purified bc1 complex | Paracoccus denitrificans | |
| 919 | - |
2 ferricytochrome c | wild type, purified bc1 complex | Paracoccus denitrificans |
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