Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.014 mM, 50% inhibition of tuna cytochrome c oxidation | Starkeya novella | |
CN- | 0.004 mM, 50% inhibition of tuna cytochrome c oxidation | Starkeya novella | |
phosphate | not with yeast cytochrome c | Starkeya novella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
ferrocytochrome c | - |
Starkeya novella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
23000 | - |
x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
32000 | - |
1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
32000 | - |
x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Triton X-100, ammonium sulfate, DEAE-cellulose, Sephadex G-150 | Starkeya novella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.4 | - |
- |
Starkeya novella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + O2 | Saccharomyces cerevisiae cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 | horse ferrocytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 | cow cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 | tuna cytochrome c | Starkeya novella | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 + H+ | - |
Starkeya novella | ferricytochrome c + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000 + x * 23000, SDS-PAGE | Starkeya novella |
dimer | 1 * 23000 + 1 * 32000, SDS-PAGE | Starkeya novella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | above 20 mM phosphate: rapid decrease of kcat with Candida krusei, tuna, Thiobacillus novellus, and horse cytochrome c | Starkeya novella | |
20 | - |
ferrocytochrome c | Thiobacillus novellus cytochrome c, 12.5 mM phosphate at pH 5.5 and 20°C | Starkeya novella | |
45.8 | - |
ferrocytochrome c | Candida krusei cytochrome c, 30 mM phosphate at pH 5.5 and 20°C | Starkeya novella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
phosphate buffer | Starkeya novella |