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Literature summary for 7.1.1.9 extracted from

  • King, M.T.; Drews, G.
    Isolation and partial characterization of the cytochrome oxidase from Rhodopseudomonas palustris (1976), Eur. J. Biochem., 68, 5-12.
    View publication on PubMed

General Stability

General Stability Organism
glycerol: no stabilization during storage Rhodopseudomonas palustris
inactivation by repeated freezing/thawing Rhodopseudomonas palustris

Inhibitors

Inhibitors Comment Organism Structure
azide 0.007 mM, 50% inhibition, 1 mM, complete inhibition Rhodopseudomonas palustris
CN- 0.0005 mM, 50% inhibition Rhodopseudomonas palustris
Lithium diiodosalicylate
-
Rhodopseudomonas palustris
N,N-Dimethyllauryl amine oxide
-
Rhodopseudomonas palustris
phosphate more than 10 mM Rhodopseudomonas palustris
sodium deoxycholate
-
Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
ferrocytochrome c oxidation of horse cytochrome c Rhodopseudomonas palustris
0.026
-
ferrocytochrome c oxidation of bovine or yeast cytochrome c Rhodopseudomonas palustris

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9500
-
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE Rhodopseudomonas palustris
12200
-
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE Rhodopseudomonas palustris
25500
-
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE Rhodopseudomonas palustris
30500
-
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE Rhodopseudomonas palustris

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50.7
-
oxidation of horse heart cytochrome c Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + O2 + H+
-
Rhodopseudomonas palustris ferricytochrome c + H2O
-
r

Subunits

Subunits Comment Organism
? x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 glycylglycine/NaOH buffer Rhodopseudomonas palustris
6
-
phosphate buffer Rhodopseudomonas palustris
7.5 8 presence of reducing agents Rhodopseudomonas palustris