Literature summary for 7.1.1.9 extracted from

Qian, J.; Shi, W.; Pressler, M.; Hoganson, C.; Mills, D.; Babcock, G.T.; Ferguson-Miller, S.
Aspartate-407 in Rhodobacter sphaeroides cytochrome c oxidase is not required for proton pumping or manganese binding (1997), Biochemistry, 36, 2539-2543.

Search for more literature for 7.1.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type, D407A, D407C and D407N mutant enzyme in Rhodobacter sphaeroides	Cereibacter sphaeroides

Protein Variants

Protein Variants	Comment	Organism
D407A	similar properties as wild type, suggesting that D407 does not play a role in proton translocating	Cereibacter sphaeroides
D407C	similar properties as wild type, suggesting that D407 does not play a role in proton translocating	Cereibacter sphaeroides
D407N	similar properties as wild type, suggesting that D407 does not play a role in proton translocating	Cereibacter sphaeroides

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	2 copper ions at the CuA site	Cereibacter sphaeroides
Mg	-	Cereibacter sphaeroides
Mn	when grown with 0.7 mM Mn2+ and 0.05 mM Mg2+, Mn2+ appears to be inserted into what is normally a Mg2+ site	Cereibacter sphaeroides

Organism

Organism	UniProt	Comment	Textmining
Cereibacter sphaeroides	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type, D407A, D407C and D407N mutant enzyme, Ni2+-nitrilotriacetic acid affinity chromatography	Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ferrocytochrome c + O2 + H+	-	Cereibacter sphaeroides	ferricytochrome c + H2O	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
550	-	ferrocytochrome c	reconstituted recombinant D407C mutant enzyme	Cereibacter sphaeroides
650	-	ferrocytochrome c	recombinant D407C mutant enzyme	Cereibacter sphaeroides
700	-	ferrocytochrome c	reconstituted recombinant D407A mutant enzyme	Cereibacter sphaeroides
700	-	ferrocytochrome c	reconstituted recombinant D407N mutant enzyme	Cereibacter sphaeroides
1100	-	ferrocytochrome c	recombinant D407A mutant enzyme	Cereibacter sphaeroides
1200	-	ferrocytochrome c	reconstituted wild-type enzyme	Cereibacter sphaeroides
1300	-	ferrocytochrome c	wild-type enzyme	Cereibacter sphaeroides
1400	-	ferrocytochrome c	recombinant D407N mutant enzyme	Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	wild-type, D407A, D407C and D407N mutant enzyme, sharp drop above	Cereibacter sphaeroides

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Release 2023.1 (January 2023)