Literature summary for 7.1.1.9 extracted from

Tsukita, S.; Koyanagi, S.; Nagata, K.; Koizuka, H.; Akashi, H.; Shimoyama, T.; Tamura, T.; Sone, N.
Characterization of a cb-type cytochrome c oxidase from Helicobacter pylori (1999), J. Biochem., 125, 194-201.

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Inhibitors

Inhibitors	Comment	Organism	Structure
CN-	-	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00004	-	O2	-	Helicobacter pylori
0.0009	-	ferrocytochrome c553	-	Helicobacter pylori

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Helicobacter pylori	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE	Helicobacter pylori
58000	-	1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE	Helicobacter pylori
200000	-	gel filtration	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Triton X-100, Q-Sepharose, chelating Sepharose, gel filtration	Helicobacter pylori

Storage Stability

Storage Stability	Organism
-80°C, no severe loss of activity	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ferrocytochrome c + O2	Saccharomyces cerevisiae cytochrome c	Helicobacter pylori	ferricytochrome c + H2O	-	?
ferrocytochrome c + O2	horse ferrocytochrome c	Helicobacter pylori	ferricytochrome c + H2O	-	?
ferrocytochrome c + O2	artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine	Helicobacter pylori	ferricytochrome c + H2O	-	?
ferrocytochrome c553 + O2 + H+	-	Helicobacter pylori	ferricytochrome c553 + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE	Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
252	-	ferrocytochrome c553	-	Helicobacter pylori

Cofactor

Cofactor	Comment	Organism	Structure
heme c	enzyme contains 3 heme c	Helicobacter pylori
protoheme	-	Helicobacter pylori

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0026	-	CN-	-	Helicobacter pylori

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Release 2023.1 (January 2023)