Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli | Streptomyces viridochromogenes |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, X-ray quality crystals are generated and the structure is solved to a resolution of 2.4 A | Streptomyces viridochromogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Streptomyces viridochromogenes | |
additional information | the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF | Streptomyces viridochromogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the recombinant protein is found in the cytoplasmic fraction | Streptomyces viridochromogenes | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | copper-containing enzyme. Contains 4 atoms per molecule of the enzyme | Streptomyces viridochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | 90000 | gel filtration | Streptomyces viridochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces viridochromogenes | J9S5G3 | - |
- |
Streptomyces viridochromogenes VKM Ac-629 | J9S5G3 | - |
- |
Storage Stability | Organism |
---|---|
20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity | Streptomyces viridochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 ferrocytochrome c + O2 + 4 H+ | - |
Streptomyces viridochromogenes | 4 ferricytochrome c + 2 H2O | - |
? | |
4 ferrocytochrome c + O2 + 4 H+ | - |
Streptomyces viridochromogenes VKM Ac-629 | 4 ferricytochrome c + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 33900, calculated from sequence | Streptomyces viridochromogenes |
homotrimer | 3 * 39000, SDS-PAGE after boiling | Streptomyces viridochromogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
20°C, 2 days, the enzyme retains about 30% residual activity | Streptomyces viridochromogenes |
3 | - |
20°C, 2 days, the enzyme retains about 42% residual activity | Streptomyces viridochromogenes |
4 | - |
20°C, 2 days, the enzyme retains about 65-60% residual activity | Streptomyces viridochromogenes |
5 | - |
20°C, 2 days, the enzyme retains about 65-60% residual activity | Streptomyces viridochromogenes |
6 | 10 | 20°C, 2 days, the enzyme retains about 70% residual activity | Streptomyces viridochromogenes |