Protein Variants | Comment | Organism |
---|---|---|
M138C | modification of epsilon-subunit, reduced inhibition of the F1 part of the enzyme EC 3.6.3.14 by the epsilon subunit | Escherichia coli |
additional information | modification of the Cys at position 10 with NEM or fluorescein maleimide further reduces the binding affinity of, and the maximal inhibition by, the epsilon subunit | Escherichia coli |
S10C | modification of epsilon-subunit, reduced inhibition of the F1 part of the enzyme EC 3.6.3.14 by the epsilon subunit | Escherichia coli |
S65C | modification of epsilon-subunit, increases inhibition of ECF1 by the epsilon subunit | Escherichia coli |
General Stability | Organism |
---|---|
F1 part of the enzyme EC 3.6.3.14 treated with trypsin at pH 7.0 still binds purified epsilon subunit, while enzyme treated with the protease at pH 8.0 does not | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | modification of the Cys at position 10 with NEM or fluorescein maleimide further reduces the binding affinity of, and the maximal inhibition by the epsilon subunit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Escherichia coli | ADP + phosphate + H+/out | - |
? | |
additional information | sites around residues 70 and/or between 202 and 212 of the gamma subunit are involved in epsilon subunit binding | Escherichia coli | ? | - |
? |