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Literature summary for 7.1.2.2 extracted from

  • Abrams, A.; Smith, J.B.
    Bacterial membrane ATPase (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 395-429.
No PubMed abstract available
Search for more literature for 7.1.2.2

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Enterococcus faecalis
Ca2+
-
 Escherichia coli
CN-
-
 Geobacillus stearothermophilus
guanidine
-
 Enterococcus faecalis
Hg2+
-
 Escherichia coli
I2
-
 Geobacillus stearothermophilus
Mg2+ Ca2+-activated enzyme Micrococcus luteus
N,N'-dicyclohexylcarbodiimide
-
 Enterococcus faecalis
N,N'-dicyclohexylcarbodiimide
-
 Escherichia coli
N3-
-
 Escherichia coli
N3-
-
 Geobacillus stearothermophilus
N3-
-
 Micrococcus luteus
N3-
-
 Priestia megaterium
phosphate
-
 Enterococcus faecalis
phosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 ATP
-
 Escherichia coli
0.9
-
 ATP strain NRLL B939 Priestia megaterium
1
-
 ATP strain KM Priestia megaterium
2.5
-
 ATP
-
 Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Alcaligenes faecalis 16020
-
membrane
-
 Escherichia coli 16020
-
membrane
-
 Geobacillus stearothermophilus 16020
-
membrane
-
 Cereibacter sphaeroides 16020
-
membrane
-
 Priestia megaterium 16020
-
membrane
-
 Enterococcus faecalis 16020
-
membrane exclusively internal location Micrococcus luteus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Geobacillus stearothermophilus
Ca2+ activates Alcaligenes faecalis
Ca2+ activates Escherichia coli
Ca2+ activates Cereibacter sphaeroides
Ca2+ activates Priestia megaterium
Ca2+ activates Enterococcus faecalis
Ca2+ activates Micrococcus luteus
Cd2+ activates Priestia megaterium
Co2+ activates Escherichia coli
Co2+ activates Geobacillus stearothermophilus
Mg2+ activates Alcaligenes faecalis
Mg2+ activates Escherichia coli
Mg2+ activates Geobacillus stearothermophilus
Mg2+ activates Cereibacter sphaeroides
Mg2+ activates Priestia megaterium
Mg2+ activates Enterococcus faecalis
Mg2+ activates Micrococcus luteus
Mg2+ absolute requirement for Mg2+ or Mn2+ Enterococcus faecalis
Mn2+ activates Escherichia coli
Mn2+ activates Geobacillus stearothermophilus
Mn2+ activates Enterococcus faecalis
Mn2+ absolute requirement for Mg2+ or Mn2+ Enterococcus faecalis
Zn2+ activates Escherichia coli
Zn2+ activates Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
51000
-
 alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
280000
-
 equilibrium sedimentation Geobacillus stearothermophilus
300000
-
 gel filtration Alcaligenes faecalis
300000
-
 gel filtration Cereibacter sphaeroides
379000
-
 strain KM, sedimentation equilibrium centrifugation Priestia megaterium
385000
-
 sedimentation equilibrium analysis Enterococcus faecalis
400000
-
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Cereibacter sphaeroides
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
-
-
Micrococcus luteus
-
-
-
Priestia megaterium
-
 and strain KM
-
Priestia megaterium NRLL B939
-
 and strain KM
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium
-
 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + H2O
-
 Enterococcus faecalis 2'-deoxy-ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Alcaligenes faecalis ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Escherichia coli ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Geobacillus stearothermophilus ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Cereibacter sphaeroides ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Priestia megaterium ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Enterococcus faecalis ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Micrococcus luteus ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Priestia megaterium NRLL B939 ADP + phosphate + H+/out
-
 ?
CTP + H2O + H+/in poorly hydrolyzed Enterococcus faecalis CDP + phosphate + H+/out
-
 ?
GTP + H2O + H+/in
-
 Enterococcus faecalis GDP + phosphate + H+/out
-
 ?
ITP + H2O + H+/in
-
 Enterococcus faecalis IDP + phosphate + H+/out
-
 ?
UTP + H2O + H+/in poorly hydrolyzed Enterococcus faecalis UDP + phosphate + H+/out
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha6,beta6, 6 * 47000 + 6 * 51000, SDS-PAGE Enterococcus faecalis
More size of major subunits Escherichia coli
More size of major subunits Priestia megaterium
More size of major subunits Enterococcus faecalis
More size of major subunits Micrococcus luteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 no cold lability Geobacillus stearothermophilus
additional information
-
 cold lability Escherichia coli
additional information
-
 cold lability Priestia megaterium
additional information
-
 cold lability Enterococcus faecalis
additional information
-
 cold lability Micrococcus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
217
-
 ATP
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Enterococcus faecalis
7.5
-
-
 Micrococcus luteus
8 8.5
-
 Geobacillus stearothermophilus
8.1
-
-
 Cereibacter sphaeroides
9 9.5
-
 Escherichia coli