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Literature summary for 7.1.2.2 extracted from

  • Stocker, A.; Keis, S.; Cook, G.M.; Dimroth, P.
    Purification, crystallization, and properties of F1-ATPase complexes from the thermoalkaliphilic Bacillus sp. strain TA2.A1 (2005), J. Struct. Biol., 152, 140-145.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Lauryldimethylamine oxide stimulates ATP hydrolysis activity of F1-wt or F1 deltasigma 30-45fold, respectively Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
F1 moiety from the atp operon into vector pTrc99A, overexpressed in Escherichia coli RNE41 in the two variant complexes F1-wt and F1 deltasigma Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
complex F1 deltasigma by micro-batch sreening, to 3.1 A resolution, no crystals obtained with complex F1-wt Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
ATP F1-wt Bacillus sp. (in: Bacteria)
0.7
-
ATP F1 deltasigma Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) TA2.A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
complexes F1-wt and F1 deltasigma, purified in high yield and purity by high affinity chromatography, anionic exchange chromatography and GPC Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
Bacillus sp. (in: Bacteria) ADP + phosphate + H+/out
-
?
ATP + H2O + H+/in
-
Bacillus sp. (in: Bacteria) TA2.A1 ADP + phosphate + H+/out
-
?

Subunits

Subunits Comment Organism
More four bands alpha, beta, gamma and epsilon in F1 deltasigma with identical molecular mass values as those in the native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
F1-ATPase
-
Bacillus sp. (in: Bacteria)