Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lauryldimethylamine oxide | stimulates ATP hydrolysis activity of F1-wt or F1 deltasigma 30-45fold, respectively | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
F1 moiety from the atp operon into vector pTrc99A, overexpressed in Escherichia coli RNE41 in the two variant complexes F1-wt and F1 deltasigma | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
complex F1 deltasigma by micro-batch sreening, to 3.1 A resolution, no crystals obtained with complex F1-wt | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
ATP | F1-wt | Bacillus sp. (in: Bacteria) | |
0.7 | - |
ATP | F1 deltasigma | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) TA2.A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
complexes F1-wt and F1 deltasigma, purified in high yield and purity by high affinity chromatography, anionic exchange chromatography and GPC | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bacillus sp. (in: Bacteria) | ADP + phosphate + H+/out | - |
? | |
ATP + H2O + H+/in | - |
Bacillus sp. (in: Bacteria) TA2.A1 | ADP + phosphate + H+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | four bands alpha, beta, gamma and epsilon in F1 deltasigma with identical molecular mass values as those in the native enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
F1-ATPase | - |
Bacillus sp. (in: Bacteria) |