Application | Comment | Organism |
---|---|---|
additional information | simple and inexpensive method to grow yeast to high density and purify the mitochondrial F1-ATPase quickly and efficiently | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
ATPase genetically modified to include a His6 Ni affinity tag on the amino end of the mature beta-subunit, imported into mitochondrion, expression of the the purified chimeric enzyme in Escherichia coli DMY301 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
by sitting drop method at 23°C, to better than 2.8 A resolution | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae DK8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-chelate affinity chromatography and gel filtration | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, overnight | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Saccharomyces cerevisiae | ADP + phosphate + H+/out | - |
r | |
ATP + H2O + H+/in | - |
Saccharomyces cerevisiae DK8 | ADP + phosphate + H+/out | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | subunits alpha, beta, gamma, delta and sigma of the purified chimeric enzyme, with the stoichiometry of 3:3:1:1:1, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
F1-ATPase | - |
Saccharomyces cerevisiae |