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Literature summary for 7.1.2.2 extracted from

  • Notari, L.; Arakaki, N.; Mueller, D.; Meier, S.; Amaral, J.; Becerra, S.P.
    Pigment epithelium-derived factor binds to cell-surface F1-ATP synthase (2010), FEBS J., 277, 2192-2205.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Protein Variants

Protein Variants Comment Organism
additional information human pigment epithelium-derived factor is immobilized on the surface of a CM5 sensor chip revealing binding response units for the yeast F1-ATPase showing specific, reversible and concentration-response binding of F1 to PEDF Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
angiostatin
-
 Bos taurus
angiostatin competes with pigment epithelium-derived factor Saccharomyces cerevisiae
pigment epithelium-derived factor
-
 Bos taurus
pigment epithelium-derived factor competes with angiostatin. Human PEDF significantly reduces the amount of extracellular ATP produced by endothelial cells, in agreement with direct interactions between cell-surface ATP synthase and PEDF, 53% inhibition at 10 nM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding kinetics of pigment epithelium-derived factor to cell surface F1-ATP synthase, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Saccharomyces cerevisiae 9986
-
cell surface
-
 Bos taurus 9986
-
plasma membrane
-
 Saccharomyces cerevisiae 5886
-
plasma membrane
-
 Bos taurus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mg2+ required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, F1-ATP synthase beta-subunit, SDS-PAGE Saccharomyces cerevisiae
60000
-
 x * 60000, F1-ATP synthase beta-subunit, SDS-PAGE Bos taurus
360000
-
 purified F1-ATP synthase, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Saccharomyces cerevisiae
-
 ADP + phosphate + H+/out
-
 r
ATP + H2O + H+/in Bos taurus
-
 ADP + phosphate + H+/out
-
 r
additional information Saccharomyces cerevisiae F1-ATP synthase beta-subunit binds specifically to the human pigment epithelium-derived factor and acts as a cell-surface receptor in retinal cells. PEDF is a ligand for endothelial cell-surface F1Fo-ATP synthase ?
-
 ?
additional information Bos taurus F1-ATP synthase beta-subunit binds to the pigment epithelium-derived factor and acts as a cell-surface receptor in retinal cells. PEDF is a ligand for endothelial cell-surface F1Fo-ATP synthase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelial cell
-
 Saccharomyces cerevisiae
-
retina
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Saccharomyces cerevisiae ADP + phosphate + H+/out
-
 r
ATP + H2O + H+/in
-
 Bos taurus ADP + phosphate + H+/out
-
 r
additional information F1-ATP synthase beta-subunit binds specifically to the human pigment epithelium-derived factor and acts as a cell-surface receptor in retinal cells. PEDF is a ligand for endothelial cell-surface F1Fo-ATP synthase Saccharomyces cerevisiae ?
-
 ?
additional information F1-ATP synthase beta-subunit binds to the pigment epithelium-derived factor and acts as a cell-surface receptor in retinal cells. PEDF is a ligand for endothelial cell-surface F1Fo-ATP synthase Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, F1-ATP synthase beta-subunit, SDS-PAGE Saccharomyces cerevisiae
? x * 60000, F1-ATP synthase beta-subunit, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
F1-ATP synthase
-
 Saccharomyces cerevisiae
F1-ATP synthase
-
 Bos taurus

General Information

General Information Comment Organism
additional information pigment epithelium-derived factor-mediated inhibition of ATP synthase may form part of the biochemical mechanisms by which PEDF exerts its antiangiogenic activity Saccharomyces cerevisiae
additional information pigment epithelium-derived factor-mediated inhibition of ATP synthase may form part of the biochemical mechanisms by which PEDF exerts its antiangiogenic activity Bos taurus