Protein Variants | Comment | Organism |
---|---|---|
alphaC193S/gammaS108C/I211C | site-directed mutagenesis | Bacillus sp. PS3 |
betaE190D | site-directed mutagenesis, mutation of the beta subunit of F1 mutant alphaC193S/gammaS108C/I211C, the mutant shows a clear pause of the temperature-sensitive reaction below 18°C, the catalytic state of the temperature-sensitive reaction in rotation of the hybrid F1, carrying a single copy of betaE190D is observed at 18°C | Bacillus sp. PS3 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus sp. PS3 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus sp. PS3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Bacillus sp. PS3 | - |
ADP + phosphate + H+/out | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. PS3 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bacillus sp. PS3 | ADP + phosphate + H+/out | - |
r | |
ATP + H2O + H+/in | the temperature-sensitive reaction is a structural rearrangement of beta subunit before or after ATP binding, overview | Bacillus sp. PS3 | ADP + phosphate + H+/out | - |
r |
Synonyms | Comment | Organism |
---|---|---|
F1-ATPase | - |
Bacillus sp. PS3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Bacillus sp. PS3 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
highly temperature-sensitive reaction step of F1-ATPase from thermophilic Bacillus PS3 below 9°C as an intervening pause before the 80° substep at the same angle for ATP binding and ADP release. Temperature dependence of the rotation in wild-type and mutant enzymes, overview | Bacillus sp. PS3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus sp. PS3 |
General Information | Comment | Organism |
---|---|---|
physiological function | F1-ATPase is a rotary molecular motor in which the gamma-subunit rotates against the alpha3beta3 cylinder. The unitary gamma-rotation is a 120° step comprising 80° and 40° substeps, each of these initiated by ATP binding and ADP release and by ATP hydrolysis and inorganic phosphate release, respectively | Bacillus sp. PS3 |