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Literature summary for 7.1.2.2 extracted from

  • Gao, X.; Mu, Z.; Yu, X.; Qin, B.; Wojdyla, J.; Wang, M.; Cui, S.
    Structural insight into conformational changes induced by ATP binding in a type III secretion-associated ATPase from Shigella flexneri (2018), Front. Microbiol., 9, 1468 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Shigella flexneri

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded enzyme or in complex with ATPgammaS, hanging drop vapor diffusion method, using 0.1 M bicine, pH 8.0, 1.4 M ammonium sulfate, 10% (w/v) ethylene glycol, at 22°C Shigella flexneri

Protein Variants

Protein Variants Comment Organism
C163V the Walker A motif mutant exhibits a similar rate of ATP hydrolysis to the wild type enzyme Shigella flexneri
D249E the mutation leads to abrogation of the ATPase activity Shigella flexneri
D308A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
D310A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
D313A the mutation leads to abrogation of the ATPase activity Shigella flexneri
E307A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
F167A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
F311A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
K165A the mutation leads to abrogation of the ATPase activity Shigella flexneri
L305A the mutation leads to abrogation of the ATPase activity Shigella flexneri
L305D the mutation leads to abrogation of the ATPase activity Shigella flexneri
L305I the mutation leads to abrogation of the ATPase activity Shigella flexneri
L306A the mutant shows reduced activity compared to the wild type enzyme Shigella flexneri
R350A the mutation leads to abrogation of the ATPase activity Shigella flexneri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Shigella flexneri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + cellular protein[side 1] Shigella flexneri
-
 ADP + phosphate + cellular protein[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Shigella flexneri P0A1C1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Hitrap Q column chromatography, and Superdex 75 gel filtration Shigella flexneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + cellular protein[side 1]
-
 Shigella flexneri ADP + phosphate + cellular protein[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
Spa47 surface presentation antigens 47 Shigella flexneri
T3SS ATPase
-
 Shigella flexneri
type III secretion-associated ATPase
-
 Shigella flexneri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 ATP at pH and °C Shigella flexneri