General Stability | Organism |
---|---|
4 M urea for 30 min does not affect activity | Sesamum indicum |
Mg2+ stabilizes enzyme | Sesamum indicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sesamum indicum | |
Ca2+ | - |
Sesamum indicum | |
Cu2+ | - |
Sesamum indicum | |
Fe3+ | - |
Sesamum indicum | |
Hg2+ | - |
Sesamum indicum | |
Zn2+ | - |
Sesamum indicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.768 | - |
diphosphate | pH 8.6, 37°C | Sesamum indicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glyoxysome | 63% of activity | Sesamum indicum | 9514 | - |
mitochondrion | 23% of activity | Sesamum indicum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slight activation | Sesamum indicum | |
Mg2+ | required, highest activity with a Mg2+/diphosphate ratio of 2 | Sesamum indicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20100 | - |
SDS-PAGE | Sesamum indicum |
20800 | - |
gel filtration | Sesamum indicum |
21900 | - |
calculated from amino acid sequence | Sesamum indicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Sesamum indicum | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate + H+[side 2] | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 70% of activity in 50% solution | Sesamum indicum |
Methanol | 70% of activity in 50% solution | Sesamum indicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sesamum indicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sesamum indicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Sesamum indicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.66 | - |
purified enzyme, pH 8.6, 37°C | Sesamum indicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Sesamum indicum | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions | Sesamum indicum | 2 phosphate + H+[side 2] | - |
? | |
additional information | trimetaphosphate and tripolyphosphate are hydrolyzed with 2% of the rate with diphosphate, no other phosphorylated compounds are hydrolyzed | Sesamum indicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | same molecular weight in SDS-PAGE and gel filtration | Sesamum indicum |
Synonyms | Comment | Organism |
---|---|---|
alkaline PPase | - |
Sesamum indicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sesamum indicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Sesamum indicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for up to 30 min | Sesamum indicum |
60 | - |
33% loss of activity within 30 min | Sesamum indicum |
80 | - |
complete loss of activity within 15 min | Sesamum indicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Sesamum indicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | - |
Sesamum indicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | stable | Sesamum indicum |