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Literature summary for 7.1.3.1 extracted from

  • Schultz, A.; Baltscheffsky, M.
    Inhibition studies on Rhodospirillum rubrum H(+)-pyrophosphatase expressed in Escherichia coli (2004), Biochim. Biophys. Acta, 1656, 156-165.
    View publication on PubMed
Search for more literature for 7.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
D204H/R206L 90% loss in proton translocating but only 50% loss in hydrolytic activity Rhodospirillum rubrum
D217A almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity Rhodospirillum rubrum
D217H almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity Rhodospirillum rubrum
H372A slight loss of activity Rhodospirillum rubrum
H372A/H702V slight loss of activity Rhodospirillum rubrum
H469R almost complete loss of hydrolytic and proton translocating activity Rhodospirillum rubrum
H652A slight loss of activity Rhodospirillum rubrum
H661A about 50% loss of activity Rhodospirillum rubrum
H702V slight loss of activity Rhodospirillum rubrum

General Stability

General Stability Organism
Mg2+/diphosphate protects against inhibition by all inhibitors in the study Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
4-bromophenacyl bromide
-
 Rhodospirillum rubrum
diethylpyrocarbonate
-
 Rhodospirillum rubrum
Fluorescein 5'-isothiocyanate
-
 Rhodospirillum rubrum
N,N'-dicyclohexyl-carbodiimide
-
 Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Rhodospirillum rubrum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Rhodospirillum rubrum important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
 ?