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Literature summary for 7.1.3.1 extracted from

  • Yang, S.J.; Jiang, S.S.; Hsiao, Y.Y.; Van, R.C.; Pan, Y.J.; Pan, R.L.
    Thermoinactivation analysis of vacuolar H(+)-pyrophosphatase (2004), Biochim. Biophys. Acta, 1656, 88-95.
    View publication on PubMed
Search for more literature for 7.1.3.1

General Stability

General Stability Organism
Mg2+/diphosphate protects against thermal inactivation Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
Fluorescein 5'-isothiocyanate
-
 Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Vigna radiata 16020
-
vacuole
-
 Vigna radiata 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 2 * 73000, SDS-PAGE Vigna radiata
145000
-
 gel filtration Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Vigna radiata important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation from cytosol to vacuole lumen 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl etiolated Vigna radiata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Vigna radiata 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation from cytosol to vacuole lumen Vigna radiata 2 phosphate + H+[side 2]
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 73000, SDS-PAGE Vigna radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 unstable above Vigna radiata