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Literature summary for 7.1.3.1 extracted from

  • Maeshima, M.; Yoshida, S.
    Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean (1989), J. Biol. Chem., 264, 20068-20073 .
    View publication on PubMed
Search for more literature for 7.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid the enzyme reaction is stimulated by exogenous phospholipid in the presence of detergent Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
ATP more than 80% inhibition at 2 mM Vigna radiata
CTP more than 80% inhibition at 2 mM Vigna radiata
diphosphate excess of diphosphate inhibits the enzyme Vigna radiata
fluoride inhibitory at high concentrations Vigna radiata
GTP more than 80% inhibition at 2 mM Vigna radiata
Mg2+ excess of Mg2+ inhibits the enzyme Vigna radiata
additional information nitrate (50 mM), molybdate (0.5 mM), and azide (2 mM) have essentially no effect on the enzyme reaction Vigna radiata
N,N'-dicyclohexylcarbodiimide
-
 Vigna radiata
vanadate inhibitory at high concentrations Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
tonoplast
-
 Vigna radiata
-
-
vacuolar membrane
-
 Vigna radiata 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ for maximum activity, the purified enzyme requires 50 mM K+ Vigna radiata
Mg2+ for maximum activity, the purified enzyme requires 1 mM Mg2+ Vigna radiata
Mn2+ slight stimulatory effect at 1 mM Mn2+ Vigna radiata
additional information Co2+ and Ca2+ have no stimulatory effect on the enzyme activity Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Vigna radiata
-
 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
 cultivar Wilczek
-

Purification (Commentary)

Purification (Comment) Organism
solubilization with lysophosphatidylcholine and QAE-Toyopearl column chromatography Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl
-
 Vigna radiata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Vigna radiata 2 phosphate + H+[side 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 73000, SDS-PAGE Vigna radiata

Synonyms

Synonyms Comment Organism
vacuolar membrane proton-translocating inorganic pyrophosphatase
-
 Vigna radiata

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2
-
 pH and temperature not specified in the publication Vigna radiata vanadate
5
-
 pH and temperature not specified in the publication Vigna radiata fluoride