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Literature summary for 7.2.1.1 extracted from

  • Fadeeva, M.S.; Bertsova, Y.V.; Verkhovsky, M.I.; Bogachev, A.V.
    Site-directed mutagenesis of conserved cysteine residues in NqrD and NqrE subunits of Na+-translocating NADH:quinone oxidoreductase (2008), Biochemistry (Moscow), 73, 123-129.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C112I NqrD-C112I. The substitutions of the conserved cysteine residue in NqrD blocks the Na+-dependent and HQNO-sensitive quinone reductase activity of the enzyme, being without effect on the interaction of the enzyme with reduced nicotinamide hypoxanthine dinucleotide and menadione. The substitution of the conserved cysteine residues results in inability of covalently bound flavins to stabilize flavosemiquinone states, i.e. leads to incorrect folding of the NQR complex Vibrio cholerae serotype O1
C120G NqrE-C120G. The substitution of the conserved cysteine residue in NqrE subunit of the enzyme blocks the Na+-dependent and HQNO-sensitive quinone reductase activity of the enzyme, being without effect on the interaction of the enzyme with reduced nicotinamide hypoxanthine dinucleotide and menadione. The substitution of the conserved cysteine residues results in inability of covalently bound flavins to stabilize flavosemiquinone states, i.e. leads to incorrect folding of the NQR complex Vibrio cholerae serotype O1
C26G NqrE-C26G. The substitution of the conserved cysteine residue in NqrE subunits of the enzyme blocks the Na+-dependent and HQNO-sensitive quinone reductase activity of the enzyme, being without effect on the interaction of the enzyme with reduced nicotinamide hypoxanthine dinucleotide and menadione. The substitution of the conserved cysteine residues results in inability of covalently bound flavins to stabilize flavosemiquinone states, i.e. leads to incorrect folding of the NQR complex Vibrio cholerae serotype O1
C29A NqrD-C29A. The substitutions of the conserved cysteine residue in NqrD subunit of the enzyme blocks the Na+-dependent and HQNO-sensitive quinone reductase activity of the enzyme, being without effect on the interaction of the enzyme with reduced nicotinamide hypoxanthine dinucleotide and menadione. The substitution of the conserved cysteine residues results in inability of covalently bound flavins to stabilize flavosemiquinone states, i.e. leads to incorrect folding of the NQR complex Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe the enzyme contains 650-780 ng Fe per mg protein, which corresponds to 2.5-3.0 atoms of Fe per enzyme complex. The enzyme bears only the 2Fe-2S cluster as the sole metal-containing prosthetic group Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1 Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
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Vibrio cholerae serotype O1 ATCC 39315 Q9KPS1 and Q9KPS2 and P0C6E0 and Q9X4Q6 and Q9X4Q7 and Q9X4Q8 Q9KPS1: subunit NqrA, Q9KPS2: subunit NqrB, P0C6E0: subunit NqrC, Q9X4Q6: subunit NqrD, Q9X4Q7: subunit NqrE, Q9X4Q8: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced nicotinamide hypoxanthine dinucleotide + menadione
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Vibrio cholerae serotype O1 oxidized nicotinamide hypoxanthine dinucleotide + menadiol
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?
reduced nicotinamide hypoxanthine dinucleotide + menadione
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Vibrio cholerae serotype O1 ATCC 39315 oxidized nicotinamide hypoxanthine dinucleotide + menadiol
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?

Synonyms

Synonyms Comment Organism
dNADH:K3 oxidoreductase
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Vibrio cholerae serotype O1
Na(+)-translocating NADH-quinone reductase subunit D
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Vibrio cholerae serotype O1
Na(+)-translocating NADH-quinone reductase subunit E
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Vibrio cholerae serotype O1
Na+-NQR
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Vibrio cholerae serotype O1
Na+-translocating NADH:quinone oxidoreductase
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Vibrio cholerae serotype O1
NADH:quinone oxidoreductase
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Vibrio cholerae serotype O1