Protein Variants | Comment | Organism |
---|---|---|
additional information | a soluble variant of NqrF lacking its hydrophobic, N-terminal helix (NqrF') is produced in Vibrio cholerae wild type and nqr deletion strain. Under identical conditions of growth and induction, the yield of NqrF' increased by 30% in the presence of the Na+-NQR. FAD-containing NqrF' species with or without the FeS cluster are observed, indicating that assembly of the FeS center, but not insertion of the flavin cofactor, is limited during overproduction in Vibrio cholerae. NqrF' lacking the [2Fe2S] cluster is less stable, partially unfolded, and therefore prone to proteolytic degradation in Vibrio cholerae | Vibrio cholerae serotype O1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Vibrio cholerae serotype O1 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the NqrF subunit contains one FAD and a [2Fe2S] cluster and catalyzes the initial oxidation of NADH. The Na+-NQR is proposed to increase the stability of NqrF' by stimulating the maturation of FeS centers | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone-1 | the NqrF subunit catalyzes the initial oxidation of NADH | Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+-NQR | - |
Vibrio cholerae serotype O1 |
Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the NqrF subunit contains one FAD and a [2Fe2S] cluster and catalyzes the initial oxidation of NADH | Vibrio cholerae serotype O1 | |
NADH | - |
Vibrio cholerae serotype O1 |