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Literature summary for 7.2.1.1 extracted from

  • Tao, M.; Fritz, G.; Steuber, J.
    The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae enhances insertion of FeS in overproduced NqrF subunit (2008), J. Inorg. Biochem., 102, 1366-1372.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information a soluble variant of NqrF lacking its hydrophobic, N-terminal helix (NqrF') is produced in Vibrio cholerae wild type and nqr deletion strain. Under identical conditions of growth and induction, the yield of NqrF' increased by 30% in the presence of the Na+-NQR. FAD-containing NqrF' species with or without the FeS cluster are observed, indicating that assembly of the FeS center, but not insertion of the flavin cofactor, is limited during overproduction in Vibrio cholerae. NqrF' lacking the [2Fe–2S] cluster is less stable, partially unfolded, and therefore prone to proteolytic degradation in Vibrio cholerae Vibrio cholerae serotype O1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Vibrio cholerae serotype O1 16020
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe the NqrF subunit contains one FAD and a [2Fe–2S] cluster and catalyzes the initial oxidation of NADH. The Na+-NQR is proposed to increase the stability of NqrF' by stimulating the maturation of FeS centers Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + ubiquinone-1 the NqrF subunit catalyzes the initial oxidation of NADH Vibrio cholerae serotype O1 NAD+ + ubiquinol-1
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Synonyms

Synonyms Comment Organism
Na+-NQR
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Vibrio cholerae serotype O1
Na+-translocating NADH:quinone oxidoreductase
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Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
FAD the NqrF subunit contains one FAD and a [2Fe–2S] cluster and catalyzes the initial oxidation of NADH Vibrio cholerae serotype O1
NADH
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Vibrio cholerae serotype O1