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Literature summary for 7.2.1.1 extracted from
- Expression and mutagenesis of the NqrC subunit of the NQR respiratory Na+ pump from Vibrio cholerae with covalently attached FMN (2001), FEBS Lett., 492, 45-49.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subunit NqrC is expressed in Vibrio cholerae and in Escherichia coli TOP10 cells. Subunit NqrB expression is toxic for Vibrio cholerae | Vibrio cholerae serotype O1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H216L | the subunit NqrC mutant contains 0.2-0.3 mol of FMN per mol of protein, the mutation reduces the FMN content (30%) of the isolated subunit NqrC | Vibrio cholerae serotype O1 |
| T225L | no covalent flavin is detected when threonine-225 is replaced by leucine, the mutation eliminates flavin binding by subunit NqrC | Vibrio cholerae serotype O1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27600 | - |
subunit NqrC, calculated from amino acid sequence | Vibrio cholerae serotype O1 |
| 32000 | - |
recombinant subunit NqrC, SDS-PAGE | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 | - |
NAD+ + ubiquinol + n Na+/out | - |
? |  |
| NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 ATCC 39541 | - |
NAD+ + ubiquinol + n Na+/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
| Vibrio cholerae serotype O1 ATCC 39541 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Sephadex G-25 gel filtration | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol + n Na+/out | - |
? | |
| NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol + n Na+/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Na+-NQR | - |
Vibrio cholerae serotype O1 |
| Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Vibrio cholerae serotype O1 | |
| FMN | subunits NqrB and NqrC contain covalently bound FMN, the wild type subunit NqrC contains approximately 1 mol of FMN per mol of protein | Vibrio cholerae serotype O1 | |
| NADH | - |
Vibrio cholerae serotype O1 | |
| riboflavin | - |
Vibrio cholerae serotype O1 | |
| [2Fe-2S]-center | - |
Vibrio cholerae serotype O1 | |
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