Literature summary for 7.2.1.2 extracted from

Chowdhury, N.P.; Klomann, K.; Seubert, A.; Buckel, W.
Reduction of flavodoxin by electron bifurcation and sodium ion-dependent reoxidation by NAD+ catalyzed by ferredoxin-NAD+ reductase (Rnf) (2016), J. Biol. Chem., 291, 11993-12002 .

Search for more literature for 7.2.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Acidaminococcus fermentans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00026	-	reduced ferredoxin [iron-sulfur] cluster	at pH 6.8, temperature not specified in the publication	Acidaminococcus fermentans
0.00042	-	reduced flavodoxin [iron-sulfur] cluster	at pH 6.8, temperature not specified in the publication	Acidaminococcus fermentans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Acidaminococcus fermentans	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	required for activity	Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	Acidaminococcus fermentans	-	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	Acidaminococcus fermentans VR4	-	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	Acidaminococcus fermentans DSM 20731	-	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir

Organism

Organism	UniProt	Comment	Textmining
Acidaminococcus fermentans	-	-	-
Acidaminococcus fermentans VR4	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans VR4	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans DSM 20731	oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans	oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans VR4	oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1]	-	Acidaminococcus fermentans DSM 20731	oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]	-	ir

Synonyms

Synonyms	Comment	Organism
Rnf complex	-	Acidaminococcus fermentans
rnfABCDEG	-	Acidaminococcus fermentans

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Acidaminococcus fermentans
NAD+	-	Acidaminococcus fermentans
[4Fe-4S]-center	-	Acidaminococcus fermentans

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Release 2023.1 (January 2023)