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Literature summary for 7.2.1.3 extracted from
- Three mammalian cytochromes b561 are ascorbate-dependent ferrireductases (2006), FEBS J., 273, 3722-3734.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-galactono-gamma-lactone | - |
Mus musculus |  |
| L-galactono-gamma-lactone | - |
Homo sapiens | |
| L-galactono-gamma-lactone | - |
Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity | Mus musculus |
| expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity | Homo sapiens |
| expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D38A | the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| E117A | the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| E196A | the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| F44A | the mutation reduces the Fe(CN)3 reductase activity by about 45% | Mus musculus |
| H105A | the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| H112A | the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| H117A | the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme | Mus musculus |
| H156A | the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme | Mus musculus |
| H47A | the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme | Mus musculus |
| H83A | the mutation completely abrogates the Fe(CN)3 reductase activity of the enzyme | Mus musculus |
| M51A | the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| N106A | the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| P48A | the mutant shows increased reductase activity compared to the wild type enzyme | Mus musculus |
| Q131A | the activity of the mutant is reduced significantly to 45% of that of the wild type | Mus musculus |
| R149A | the mutation results in a 75% loss in activity compared to the wild type enzyme | Mus musculus |
| R67A | the mutation results in an almost complete loss of the Fe(CN)3 reductase activity | Mus musculus |
| S115A | the ferrireductase activity in the mutant is reduced to 50% | Mus musculus |
| W119A | the ferrireductase activity in the mutant is reduced to 17% | Mus musculus |
| W119A | the mutant shows decreased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| Y190A | the mutant shows increased Fe(CN)3 reductase activity compared to the wild type enzyme | Mus musculus |
| Y66A | the mutation results in an almost complete loss of the Fe(CN)3 reductase activity | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromaffin granule | - |
Mus musculus | 42583 | - |
| chromaffin granule | - |
Homo sapiens | 42583 | - |
| chromaffin granule | - |
Rattus norvegicus | 42583 | - |
| lysosome | - |
Mus musculus | 5764 | - |
| lysosome | - |
Homo sapiens | 5764 | - |
| lysosome | - |
Rattus norvegicus | 5764 | - |
| plasma membrane | - |
Mus musculus | 5886 | - |
| plasma membrane | - |
Homo sapiens | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
LCytb, SDS-PAGE | Mus musculus |
| 27800 | - |
CGCytb, SDS-PAGE | Mus musculus |
| 31500 | - |
DCytb, SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| duodenum | - |
Mus musculus | - |
| duodenum | - |
Homo sapiens | - |
| duodenum | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ascorbate[side 1] + Fe(CN)3[side 2] | - |
Mus musculus | monodehydroascorbate[side 1] + ? | - |
? | |
| ascorbate[side 1] + Fe(CN)3[side 2] | - |
Homo sapiens | monodehydroascorbate[side 1] + ? | - |
? | |
| ascorbate[side 1] + Fe(CN)3[side 2] | - |
Rattus norvegicus | monodehydroascorbate[side 1] + ? | - |
? | |
| ascorbate[side 1] + Fe3+-EDTA[side 2] | - |
Mus musculus | monodehydroascorbate[side 1] + ? | - |
? | |
| ascorbate[side 1] + Fe3+-EDTA[side 2] | - |
Homo sapiens | monodehydroascorbate[side 1] + ? | - |
? | |
| ascorbate[side 1] + Fe3+-EDTA[side 2] | - |
Rattus norvegicus | monodehydroascorbate[side 1] + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ascorbate-dependent ferrireductase | - |
Mus musculus |
| ascorbate-dependent ferrireductase | - |
Homo sapiens |
| ascorbate-dependent ferrireductase | - |
Rattus norvegicus |
| CGCytb | - |
Mus musculus |
| CGCytb | - |
Homo sapiens |
| CGCytb | - |
Rattus norvegicus |
| chromaffin granule cytochrome b | - |
Mus musculus |
| chromaffin granule cytochrome b | - |
Homo sapiens |
| chromaffin granule cytochrome b | - |
Rattus norvegicus |
| Cyt b561 | - |
Mus musculus |
| Cyt b561 | - |
Homo sapiens |
| Cyt b561 | - |
Rattus norvegicus |
| DCytb | - |
Mus musculus |
| DCytb | - |
Homo sapiens |
| duodenal cytochrome b | - |
Mus musculus |
| duodenal cytochrome b | - |
Homo sapiens |
| LCytb | - |
Mus musculus |
| LCytb | - |
Homo sapiens |
| LCytb | - |
Rattus norvegicus |
| lysosomal cytochrome b | - |
Mus musculus |
| lysosomal cytochrome b | - |
Homo sapiens |
| lysosomal cytochrome b | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | dependent on | Mus musculus | |
| ascorbate | dependent on | Homo sapiens | |
| ascorbate | dependent on | Rattus norvegicus | |
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