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Literature summary for 7.2.2.12 extracted from

  • Sharma, R.; Rensing, C.; Rosen, B.P.; Mitra, B.
    The ATP hydrolytic activity of purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase from Escherichia coli (2000), J. Biol. Chem., 275, 3873-3878.
    View publication on PubMed
Search for more literature for 7.2.2.12

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ in absence of thiolates Cd(II) inhibits above pH 6 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 Zn2+
-
 Escherichia coli
0.0069
-
 Pb2+
-
 Escherichia coli
0.109
-
 Zn(thiolate)2
-
 Escherichia coli
0.115
-
 Cd(thiolate)2
-
 Escherichia coli
0.118
-
 Pb(thiolate)2
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd(thiolate)2 [side 1]
-
 Escherichia coli ADP + phosphate + Cd(thiolate)2 [side 2]
-
 ?
ATP + H2O + Cd2+/in highest activity when the metal is present as thiolate complex with Cys or glutathione Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Hg2+/in
-
 Escherichia coli ADP + phosphate + Hg2+/out
-
 ?
ATP + H2O + Pb(thiolate)2 [side 1]
-
 Escherichia coli ADP + phosphate + Pb(thiolate)2 [side 2]
-
 ?
ATP + H2O + Pb2+/in highest activity when the metal is present as thiolate complex with Cys or glutathione Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn(thiolate)2 [side 1]
-
 Escherichia coli ADP + phosphate + Zn(thiolate)2 [side 2]
-
 ?
ATP + H2O + Zn2+/in highest activity when the metal is present as thiolate complex with Cys or glutathione Escherichia coli ADP + phosphate + Zn2+/out
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 Cd(II)-dependent activity Escherichia coli
6 6.5 Pb(II)-dependent and Zn(II)-dependent activity Escherichia coli
7
-
 Pb(thiolate)2, Cd(thiolate)2, Zn(thiolate)2 Escherichia coli