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Literature summary for 7.2.2.8 extracted from

  • Mandal, A.K.; Yang, Y.; Kertesz, T.M.; Argueello, J.M.
    Identification of the transmembrane metal binding site in Cu+-transporting PIB-type ATPases (2004), J. Biol. Chem., 279, 54802-54807.
    View publication on PubMed
Search for more literature for 7.2.2.8

Protein Variants

Protein Variants Comment Organism
I685E Cu+/ATPase activity is about 50% of the activity of the wild-type enzyme Archaeoglobus fulgidus
I685T Cu+/ATPase activity is about 25% of the activity of the wild-type enzyme Archaeoglobus fulgidus
L686A Cu+/ATPase activity is about 80% of the activity of the wild-type enzyme Archaeoglobus fulgidus
M711A no Cu+/ATPase activity Archaeoglobus fulgidus
M711C no Cu+/ATPase activity Archaeoglobus fulgidus
additional information Tyr682, Asn683, Met711 and Ser715 are identified as required for Cu+ binding. Replacement of these residues abolishes enzyme activity. These mutant proteins do not undergo Cu+-dependent phosphorylation by ATP but are phosphorylated by inorganic phosphate in the absence of Cu+ Archaeoglobus fulgidus
N683A no Cu+/ATPase activity Archaeoglobus fulgidus
N683Q no Cu+/ATPase activity Archaeoglobus fulgidus
P688A Cu+/ATPase activity is about 70% of the activity of the wild-type enzyme Archaeoglobus fulgidus
P704A Cu+/ATPase activity is about 75% of the activity of the wild-type enzyme Archaeoglobus fulgidus
S714A Cu+/ATPase activity is about 30% of the activity of the wild-type enzyme Archaeoglobus fulgidus
S714R Cu+/ATPase activity is about 65% of the activity of the wild-type enzyme Archaeoglobus fulgidus
S715A no Cu+/ATPase activity Archaeoglobus fulgidus
S715R no Cu+/ATPase activity Archaeoglobus fulgidus
Y682A no Cu+/ATPase activity Archaeoglobus fulgidus
Y682S Cu+/ATPase activity is about 10% of the activity of the wild-type enzyme Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu+[side 1] Tyr682, Asn683, Met711 and Ser715 are identified as required for Cu+ binding Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
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 ?

Synonyms

Synonyms Comment Organism
CopA
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 Archaeoglobus fulgidus
Cu+-transporting ATPase
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 Archaeoglobus fulgidus