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Literature summary for 7.2.2.8 extracted from

  • Gonzalez-Guerrero, M.; Hong, D.; Argueello, J.M.
    Chaperone-mediated Cu+ delivery to Cu+ transport ATPases: requirement of nucleotide binding (2009), J. Biol. Chem., 284, 20804-20811.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Archaeoglobus fulgidus
expression in Escherichia coli Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
C382A the mutant exhibits reduced copper binding activity Archaeoglobus fulgidus
M711A the mutant exhibits reduced copper binding activity Archaeoglobus fulgidus
S715A the mutant exhibits reduced copper binding activity Archaeoglobus fulgidus
Y682A the mutant exhibits reduced copper binding activity Archaeoglobus fulgidus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Archaeoglobus fulgidus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu+ the enzyme contains 2.2 Cu+ and 3.1 ions Cu+ per molecule in the absence and presence of 2.5 mM ADP-Mg2+, respectively Archaeoglobus fulgidus
Mg2+ required, bound to ATP Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu+[side 1] Archaeoglobus fulgidus
-
 ADP + phosphate + Cu+[side 2]
-
 ?
ATP + H2O + Cu+[side 1] Archaeoglobus fulgidus the enzyme drives the efflux of Cu+ from the cell cytoplasm ADP + phosphate + Cu+[side 2]
-
 ?
additional information Archaeoglobus fulgidus in absence of other ligands, CopZ transfers Cu+ to the wild type enzyme CopA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Archaeoglobus fulgidus O29777
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu+[side 1]
-
 Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
-
 ?
ATP + H2O + Cu+[side 1] the enzyme drives the efflux of Cu+ from the cell cytoplasm Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
-
 ?
ATP + H2O + Cu+[side 1] mechanism of Cu+ transfer Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
-
 ?
additional information in absence of other ligands, CopZ transfers Cu+ to the wild type enzyme CopA Archaeoglobus fulgidus ?
-
 ?

Synonyms

Synonyms Comment Organism
CopA
-
 Archaeoglobus fulgidus
Cu+ transport ATPase
-
 Archaeoglobus fulgidus