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Literature summary for 7.2.2.8 extracted from

  • Roman, E.A.; Argüello, J.M.; Gonzalez Flecha, F.L.
    Reversible unfolding of a thermophilic membrane protein in phospholipid/detergent mixed micelles (2010), J. Mol. Biol., 397, 550-559.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl the incubation of the enzyme reconstituted in phospholipid/detergent mixed micelles with high concentrations of guanidinium hydrochloride at 25°C induces a reversible decrease in fluorescence quantum yield, far-UV ellipticity, and loss of ATPase and phosphatase activities. Refolding of the enzyme from this unfolded state leads to recovery of full biological activity and all the structural features of the native enzyme. The unfolding shows typical characteristics of a two-state process. Most of the secondary and tertiary structures are disrupted. The fraction of Trp fluorescence accessible to soluble quenchers shifts from 0.52 in the native state to 0.96 in the unfolded state, with a significant spectral redshift. Hydrophobic patches in the enzyme, mainly located in the transmembrane region, are disrupted as indicated by 1-anilino-naphtalene-8-sulfonate fluorescence. Nevertheless, the unfolded state has a small but detectable amount of residual structure, which might play a key role in both CopA folding and adaptation for working at high temperatures Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29777
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
CopA
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
assay at Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
assay Archaeoglobus fulgidus

General Information

General Information Comment Organism
physiological function the enzyme is involved in transporting Cu+ throughout biological membranes Archaeoglobus fulgidus