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Literature summary for 7.6.2.11 extracted from
- Polyamine transport in bacteria and yeast (1999), Biochem. J., 344, 633-642.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| PotF-putrescine complex | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dissociation constant of spermidine and putrescine of the spermidine-preferential uptake system in presence of 1 mM Mg2+ and 100 mM K+ at pH 7.5 are 0.0032 mM and 0.1 mM | Escherichia coli | |
| 0.385 | - |
ATP | ATPase activity of PotA | Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | PotB and PotC contain six putative transmembrane segments linked by hydrophobic segments of variable length | Escherichia coli | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
| Borreliella burgdorferi | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Haemophilus influenzae | - |
- |
- |
| Mycoplasma genitalium | - |
- |
- |
| Mycoplasma pneumoniae | - |
- |
- |
| Treponema pallidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PotD protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the following amino acids of the PotD protein are involved in binding of spermidine: Trp34, Thr35, Glu36, Tyr37, Ser83, Tyr85, Asp168, Glu171, Trp229 | Escherichia coli | ? | - |
? | |
| additional information | PotA protein is involved in the energy-coupling step | Escherichia coli | ? | - |
? | |
| putrescine/out + ATP + H2O | two polyamine-uptake systems: one is a putrescine-specific system and the other is a spermidine-preferential system | Escherichia coli | putrescine/in + ADP + phosphate | - |
? | |
| spermidine/out + ADP + H2O | the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP | Escherichia coli | spermidine/in + ? | - |
? | |
| spermidine/out + ATP + H2O | two polyamine-uptake systems: one is a putrescine-specific system and the other is a spermidine-preferential system.The nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP | Escherichia coli | spermidine/in + ADP + phosphate | - |
? | |
| spermidine/out + CTP + H2O | the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP | Escherichia coli | spermidine/in + CDP + phosphate | - |
? | |
| spermidine/out + GTP + H2O | the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP | Escherichia coli | spermidine/in + GDP + phosphate | - |
? | |
| spermidine/out + UTP + H2O | the nucleotide specificity of PotA from the spermidine-preferential system in the order of decreasing activity: ATP, GTP = ADP, CTP = UTP | Escherichia coli | spermidine/in + UDP + phosphate | - |
? | |
| spermine/out + ATP + H2O | two polyamine-uptake systems: one is a putrescine-specific system and the other is a spermidine-preferential system | Escherichia coli | spermine/in + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the spermidine-preferential uptake system consists of 4 proteins, that are all necessary for spermidine uptake: PotA, PotB, PotC and PotD. The calculated molecular weights are 43000 Da for PotA, 31000 Da for PotB, 29000 Da for PotC and 39000 Da for PotD. The putrescine-uptake system consists of the 4 proteins: PotF, PotG, PotH and PotI. All 4 proteins are necessary for maximal putrescine transport. PotF is a 38000 Da putrescine binding protein, PotG is a 45000 Da membrane-associated ATPase, PotH and PotI are 31000-35000 Da channel-forming proteins | Escherichia coli |
| More | the enzyme is a ATP-binding cassette transporter | Haemophilus influenzae |
| More | the enzyme is a ATP-binding cassette transporter | Mycoplasma genitalium |
| More | the enzyme is a ATP-binding cassette transporter | Escherichia coli |
| More | the enzyme is a ATP-binding cassette transporter | Archaeoglobus fulgidus |
| More | the enzyme is a ATP-binding cassette transporter | Mycoplasma pneumoniae |
| More | the enzyme is a ATP-binding cassette transporter | Treponema pallidum |
| More | the enzyme is a ATP-binding cassette transporter | Borreliella burgdorferi |
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