BRENDA - Enzyme Database

Stereochemical applications of the expression of the L-2,3-butanediol dehydrogenase gene in Escherichia coli

Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Mimura, A.; Ohkuma, M.; Kudo, T.; Lett. Appl. Microbiol. 32, 93-98 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.76
expression in Escherichia coli JM109, stereospecifity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecifity is exhibited
Corynebacterium glutamicum
1.1.1.76
When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
Corynebacterium glutamicum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.76
Corynebacterium glutamicum
-
C-1012
-
1.1.1.76
Corynebacterium glutamicum C-1012
-
C-1012
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.76
additional information
-
highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid
Corynebacterium glutamicum
1.1.1.76
1.51
-
plasmid containing L-butanediol dehydrogenase gene
Corynebacterium glutamicum
1.1.1.76
17
-
plasmid containing tac promoter and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
1.1.1.76
18.6
-
plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.76
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
1.1.1.76
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
1.1.1.76
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum
(S,S)-butanediol + NAD+
-
-
-
?
1.1.1.76
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum C-1012
(S,S)-butanediol + NAD+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
1.1.1.76
NADH
-
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.76
expression in Escherichia coli JM109, stereospecifity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecifity is exhibited
Corynebacterium glutamicum
1.1.1.76
When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
Corynebacterium glutamicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
1.1.1.76
NADH
-
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.76
additional information
-
highest specific activities without glucose addition to culture medium, induction with 0.25 mg/ml isopropyl-beta-thiogalactopyranosid
Corynebacterium glutamicum
1.1.1.76
1.51
-
plasmid containing L-butanediol dehydrogenase gene
Corynebacterium glutamicum
1.1.1.76
17
-
plasmid containing tac promoter and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
1.1.1.76
18.6
-
plasmid containing meso-butanediol dehydrogenase promoter region and L-butanediol dehydrogenase ORF
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.76
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum
L-2,3-butanediol
-
389578
Corynebacterium glutamicum
r
1.1.1.76
L-acetoin + NADH
short chain dehydrogenase reductase family
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
1.1.1.76
L-acetoin + NADH
exhibits marked sequence similarity and common functionally conserved sequence with meso-enzyme
389578
Corynebacterium glutamicum C-1012
L-2,3-butanediol
-
389578
Corynebacterium glutamicum C-1012
r
1.1.1.76
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum
(S,S)-butanediol + NAD+
-
-
-
?
1.1.1.76
L-acetoin + NADH + H+
To confirm the high production of enzyme, the conversion of L-acetoin, in a racemic mixture, to L-2,3-butanediol is studied. 0.37% L-2,3-butanediol is formed from 1% L-acetoin added to the culture.
389578
Corynebacterium glutamicum C-1012
(S,S)-butanediol + NAD+
-
-
-
?