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Literature summary extracted from

  • Mizugaki, M.; Nishimaki, T.; Shiraishi, T.; Kawaguchi, A.; Okuda, S.; Yamanaka, H.
    Studies on the metabolism of unsaturated fatty acids. IX. Stereochemical studies of the reaction catalyzed by trans-2-enoyl-coenzyme A reductase of Escherichia coli (1982), J. Biochem., 92, 1649-1654.
    View publication on PubMed


EC Number Organism UniProt Comment Textmining Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism partial Escherichia coli


EC Number Reaction Comment Organism Reaction ID acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH + H+ mechanism: anti addition of hydrogen via a 2Re, 3Re attack on trans double bond Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac. trans-2-octenoyl-CoA + NADPH
Escherichia coli octanoyl-CoA + NADP+

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism 25
assay at Escherichia coli


EC Number Cofactor Comment Organism Structure NADPH
Escherichia coli