BRENDA - Enzyme Database

Thiol-disulfide transhydrogenase (yeast)

Nagai, S.; Methods Enzymol. 17B, 510-515 (1971)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.8.4.4
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
1.8.4.4
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.4
cell associated
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.4
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.8.4.4
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.8.4.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.4
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
1.8.4.4
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.8.4.4
cystine + GSH
-
395015
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
1.8.4.4
additional information
inactive with: insulin and other proteins
395015
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395015
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.4.4
54
-
crude state, quite labile to heat above 54°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.4
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
1.8.4.4
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
1.8.4.4
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.4
cell associated
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.4
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.4
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.4.4
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.8.4.4
cystine + GSH
-
395015
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
1.8.4.4
additional information
inactive with: insulin and other proteins
395015
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.4
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395015
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.4.4
54
-
crude state, quite labile to heat above 54°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.4
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae