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Literature summary extracted from

  • Bell, A.F.; Stratton, C.F.; Zhang, X.; Novichenok, P.; Jaye, A.A.; Nair, P.A.; Parikh, S.; Rawat, R.; Tonge, P.J.
    Evidence from Raman spectroscopy that InhA, the mycobacterial enoyl reductase, modulates the conformation of the NADH cofactor to promote catalysis (2007), J. Am. Chem. Soc., 129, 6425-6431.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.38 expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.38 F149A the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.38 0.00433
-
 2-dodecenoyl-CoA mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
1.3.1.38 0.0054
-
 2-dodecenoyl-CoA wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
1.3.1.38 0.0152
-
 NADH mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
1.3.1.38 0.0352
-
 NADH wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.38 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.38 2-dodecenoyl-acyl carrier protein + NADH
-
 Mycobacterium tuberculosis dodecanoyl-acyl carrier protein + NAD+
-
 ?
1.3.1.38 2-trans,4-trans-decadienoyl-CoA + NADPH + H+
-
 Mycobacterium tuberculosis (4E)-dec-4-enoyl-CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.38 enoyl reductase
-
 Mycobacterium tuberculosis
1.3.1.38 InhA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.38 0.11
-
 (4E)-dec-4-enoyl-CoA mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
1.3.1.38 3.17
-
 (4E)-dec-4-enoyl-CoA wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.38 NADH
-
 Mycobacterium tuberculosis