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Literature summary extracted from

  • Koeduka, T.; Fridman, E.; Gang, D.; Vassao, D.; Jackson, B.; Kish, C.; Orlova, I.; Spassova, S.; Lewis, N.; Noel, J.; Baiga, T.; Dudareva, N.; Pichersky, E.
    Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester (2006), Proc. Natl. Acad. Sci. USA, 103, 10128-10133.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.318 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative expression analysis Ocimum basilicum
1.1.1.319 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative expression analysis Petunia x hybrida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+ Ocimum basilicum
-
eugenol + acetate + NADP+
-
?
1.1.1.319 coniferyl acetate + NADPH + H+ Petunia x hybrida
-
isoeugenol + acetate + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Ocimum basilicum Q15GI4
-
-
1.1.1.319 Petunia x hybrida Q15GI3 cv. Mitchell
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.318 eugenol + a carboxylate + NADP+ = a coniferyl ester + NADPH + H+ reaction mechanism, overview Ocimum basilicum
1.1.1.319 isoeugenol + acetate + NADP+ = coniferyl acetate + NADPH + H+ reaction mechanism, overview Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.318 glandular trichome
-
Ocimum basilicum
-
1.1.1.319 flower
-
Petunia x hybrida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+
-
Ocimum basilicum eugenol + acetate + NADP+
-
?
1.1.1.318 coniferyl acetate + NADPH + H+
-
Ocimum basilicum eugenol + acetate + NADP+ GC-MS analysis product analysis ?
1.1.1.319 coniferyl acetate + NADPH + H+
-
Petunia x hybrida isoeugenol + acetate + NADP+
-
?
1.1.1.319 coniferyl acetate + NADPH + H+
-
Petunia x hybrida isoeugenol + acetate + NADP+ GC-MS analysis product analysis ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.318 EGS
-
Ocimum basilicum
1.1.1.318 EGS1
-
Ocimum basilicum
1.1.1.318 eugenol synthase 1
-
Ocimum basilicum
1.1.1.319 IGS
-
Petunia x hybrida
1.1.1.319 IGS1
-
Petunia x hybrida
1.1.1.319 isoeugenol synthase 1
-
Petunia x hybrida

Temperature Optimum [┬░C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.318 28
-
assay at Ocimum basilicum
1.1.1.319 28
-
assay at Petunia x hybrida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.318 6.5
-
assay at Ocimum basilicum
1.1.1.319 6.5
-
assay at Petunia x hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.318 NADPH
-
Ocimum basilicum
1.1.1.319 NADPH
-
Petunia x hybrida

General Information

EC Number General Information Comment Organism
1.1.1.318 evolution the phenylpropene-forming eugenol synthase, EGS, belongs to a structural family of NADPH-dependent reductases that also includes isoeugenol synthase, IGS, pinoresinol-lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview Ocimum basilicum
1.1.1.319 evolution the phenylpropene-forming isoeugenol, IGS, synthase belongs to a structural family of NADPH-dependent reductases that also includes eugenol synthase, EGS, pinoresinol–lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview Petunia x hybrida