Literature summary extracted from

Milne, N.; Wahl, S.; van Maris, A.; Pronk, J.; Daran, J.
Excessive by-product formation: A key contributor to low isobutanol yields of engineered (2016), Metab. Eng. Commun., 3, 39-51.

Application

EC Number	Application	Comment	Organism
1.1.1.383	biofuel production	a cytosolically located, cofactor-balanced isobutanol pathway, consisting of a mosaic of bacterial enzymes is expressed in Sacchaormyces cerevisiae. In aerobic cultures, the pathway intermediate isobutyraldehyde is oxidized to isobutyrate rather than reduced to isobutanol. Significant concentrations of the pathway intermediates 2,3-dihydroxyisovalerate and alpha-ketoisovalerate, as well as diacetyl and acetoin, accumulate extracellularly. While the engineered strain cannot grow anaerobically, micro-aerobic cultivation results in isobutanol formation at a yield of 0.018 mol/mol glucose. Simultaneously, 2,3-butanediol is produced at a yield of 0.64 mol/molglucose	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.383	Escherichia coli	P05793	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.383	ilvC	-	Escherichia coli

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Release 2023.1 (January 2023)