BRENDA - Enzyme Database

Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl

Wang, Y.; Li, L.; Ma, C.; Gao, C.; Tao, F.; Xu, P.; Sci. Rep. 3, 2643 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.76
co-expressed with glucose dehydrogenase and formate dehydrogenase in Escherichia coli BL21(DE3) cells
Corynebacterium glutamicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.76
26800
-
x * 26800, SDS-PAGE
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.76
(2S,3S)-butane-2,3-diol + NAD+
Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.76
Corynebacterium glutamicum
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.76
39
-
at pH 7.4 and 30░C
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.76
(2S)-acetoin + NADH + H+
-
739652
Corynebacterium glutamicum
(2S,3S)-butane-2,3-diol + NAD+
-
-
-
r
1.1.1.76
(2S,3S)-butane-2,3-diol + NAD+
-
739652
Corynebacterium glutamicum
(2S)-acetoin + NADH + H+
-
-
-
r
1.1.1.76
diacetyl + NADH + H+
-
739652
Corynebacterium glutamicum
(2S)-acetoin + NAD+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.76
?
x * 26800, SDS-PAGE
Corynebacterium glutamicum
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.76
2,3-BDH
-
Corynebacterium glutamicum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.76
co-expressed with glucose dehydrogenase and formate dehydrogenase in Escherichia coli BL21(DE3) cells
Corynebacterium glutamicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.76
NAD+
-
Corynebacterium glutamicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.76
26800
-
x * 26800, SDS-PAGE
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.76
(2S,3S)-butane-2,3-diol + NAD+
Corynebacterium glutamicum
-
(2S)-acetoin + NADH + H+
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.76
39
-
at pH 7.4 and 30░C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.76
(2S)-acetoin + NADH + H+
-
739652
Corynebacterium glutamicum
(2S,3S)-butane-2,3-diol + NAD+
-
-
-
r
1.1.1.76
(2S,3S)-butane-2,3-diol + NAD+
-
739652
Corynebacterium glutamicum
(2S)-acetoin + NADH + H+
-
-
-
r
1.1.1.76
diacetyl + NADH + H+
-
739652
Corynebacterium glutamicum
(2S)-acetoin + NAD+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.76
?
x * 26800, SDS-PAGE
Corynebacterium glutamicum