BRENDA - Enzyme Database

Membrane-bound D-lactate dehydrogenase from Escherichia coli: Purification and properties

Pratt, E.; Fung, L.; Flower, J.; Ho, C.; Biochemistry 18, 312-316 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.12
Triton X-100
5fold increase in activtiy of purified detergent-free enzyme
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.4
-
(R)-lactate
pH 8.0, 23C
Escherichia coli
1.1.5.12
0.4
-
(R)-lactate
presence of Triton X-100, pH 8.0, 23C
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.12
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.5.12
Escherichia coli
-
-
-
1.1.5.12
Escherichia coli W3110trpA33
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.5.12
from sonicated cell extracts, purifiaction in the presence of 1% Triton X-100 and 0.1% sodium dodecyl sulfate
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.5.12
230
-
pH 8.0, 23C
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.1.5.12
-20C, detergent-free enzyme is destroyed by freezing and thawing
Escherichia coli
1.1.5.12
4C, stable up to 6 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.5.12
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
1.1.5.12
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli W3110trpA33
pyruvate + reduced phenazine methosulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.12
?
x * 75000, SDS-PAGE
Escherichia coli
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.5.12
60
-
1 h, 35% loss of activity, more readily inactivated in presence of Triton X-100
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
9
9.5
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
visible absorption spectrum of the purified enzyme shows a broad peak at around 450 nm and a shoulder at around 480 nm
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.12
Triton X-100
5fold increase in activtiy of purified detergent-free enzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
visible absorption spectrum of the purified enzyme shows a broad peak at around 450 nm and a shoulder at around 480 nm
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.4
-
(R)-lactate
pH 8.0, 23C
Escherichia coli
1.1.5.12
0.4
-
(R)-lactate
presence of Triton X-100, pH 8.0, 23C
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.12
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.12
from sonicated cell extracts, purifiaction in the presence of 1% Triton X-100 and 0.1% sodium dodecyl sulfate
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.5.12
230
-
pH 8.0, 23C
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.5.12
-20C, detergent-free enzyme is destroyed by freezing and thawing
Escherichia coli
1.1.5.12
4C, stable up to 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.5.12
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli
pyruvate + reduced phenazine methosulfate
-
-
-
?
1.1.5.12
(R)-lactate + oxidized phenazine methosulfate
-
740029
Escherichia coli W3110trpA33
pyruvate + reduced phenazine methosulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.12
?
x * 75000, SDS-PAGE
Escherichia coli
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.5.12
60
-
1 h, 35% loss of activity, more readily inactivated in presence of Triton X-100
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
9
9.5
-
Escherichia coli