BRENDA - Enzyme Database

Mechanisms of active transport in isolated bacterial membrane vesicles

Kohn, L.; Kaback, H.; J. Biol. Chem. 248, 7012-7017 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.12
2-hydroxy-3-butynoic acid
inactivation
Escherichia coli
1.1.5.12
additional information
not inhibited by 4-chloromercuribenzoate, N-ethylmaleimide, or 5,5'-dithiobis(2-nitrobenzoic acid)
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.5
-
D-lactate
presence of Triton X-100, pH 7.8, 25°C
Escherichia coli
1.1.5.12
1.4
-
D-lactate
pH 7.8, 25°C
Escherichia coli
1.1.5.12
21
-
L-lactate
presence of Triton X-100, pH 7.8, 25°C
Escherichia coli
1.1.5.12
30
-
L-lactate
pH 7.8, 25°C
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.5.12
Escherichia coli
-
-
-
1.1.5.12
Escherichia coli ML 308-225
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.5.12
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.5.12
D-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
D-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
740643
Escherichia coli
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
?
1.1.5.12
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
?
1.1.5.12
L-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
L-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
additional information
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
740643
Escherichia coli
?
-
-
-
?
1.1.5.12
additional information
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
740643
Escherichia coli ML 308-225
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.12
?
x * 75000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
7.5
8
-
Escherichia coli
1.1.5.12
8
9.5
presence of Triton X-100
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
1 mol of FAD per mol of enzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.12
FAD
1 mol of FAD per mol of enzyme
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.12
2-hydroxy-3-butynoic acid
inactivation
Escherichia coli
1.1.5.12
additional information
not inhibited by 4-chloromercuribenzoate, N-ethylmaleimide, or 5,5'-dithiobis(2-nitrobenzoic acid)
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.12
0.5
-
D-lactate
presence of Triton X-100, pH 7.8, 25°C
Escherichia coli
1.1.5.12
1.4
-
D-lactate
pH 7.8, 25°C
Escherichia coli
1.1.5.12
21
-
L-lactate
presence of Triton X-100, pH 7.8, 25°C
Escherichia coli
1.1.5.12
30
-
L-lactate
pH 7.8, 25°C
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.12
75000
-
x * 75000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.12
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.5.12
D-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
D-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
740643
Escherichia coli
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
?
1.1.5.12
D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
-
-
?
1.1.5.12
L-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
L-lactate + 2,6-dichloroindophenol
-
740643
Escherichia coli ML 308-225
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
1.1.5.12
additional information
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
740643
Escherichia coli
?
-
-
-
?
1.1.5.12
additional information
not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate
740643
Escherichia coli ML 308-225
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.12
?
x * 75000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.12
7.5
8
-
Escherichia coli
1.1.5.12
8
9.5
presence of Triton X-100
Escherichia coli