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Literature summary extracted from

  • Dhar, N.; Sarangapani, S.; Reddy, V.A.; Kumar, N.; Panicker, D.; Jin, J.; Chua, N.H.; Sarojam, R.
    Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis (2020), J. Exp. Bot., 71, 3638-3652 .
    View publication on PubMedView publication on EuropePMC


EC Number Cloned (Comment) Organism gene EGS1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3). Transient coexpression of ObCAAT1 and/o ObCAAT2 with ObEGS1 under control of CMV 35S promoter in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection, the transgenic leaves produce eugenol only when external coniferyl alcohol is added Ocimum basilicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac. coniferyl acetate + NADPH + H+ Ocimum basilicum
eugenol + acetate + NADP+


EC Number Organism UniProt Comment Textmining Ocimum basilicum Q15GI4

Purification (Commentary)

EC Number Purification (Comment) Organism recombinant GST-tagged enzyme EGS1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining leaf in leaves, not in leaf stripped of PGTs Ocimum basilicum
- additional information no expression of EGS1 in roots Ocimum basilicum
- peltate glandular trichome PGTs Ocimum basilicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac. coniferyl acetate + NADPH + H+
Ocimum basilicum eugenol + acetate + NADP+


EC Number Subunits Comment Organism ? x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Ocimum basilicum


EC Number Synonyms Comment Organism EGS1
Ocimum basilicum eugenol synthase 1
Ocimum basilicum ObEGS1
Ocimum basilicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism 22
assay at room temperature Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism 6
assay at Ocimum basilicum


EC Number Cofactor Comment Organism Structure NADP+
Ocimum basilicum NADPH
Ocimum basilicum

General Information

EC Number General Information Comment Organism metabolism PGT-enriched BAHD acyltransferases, encoded by genes ObCAAT1 and ObCAAT2, convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis, followed by reduction of coniferyl acetate to eugenol and acetate catalyzed by eugenol synthase EGS1. Both ObCAAT1 and ObCAAT2 in combination with ObEGS1 produce eugenol The phenylpropene biosynthesis is not coupled to lignification in sweet basil. Plant phenylpropanoid pathway, overview Ocimum basilicum additional information ObCAAT1-RNAi transgenic lines show decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives Ocimum basilicum physiological function sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase Ocimum basilicum