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Literature summary extracted from

  • Dhar, N.; Sarangapani, S.; Reddy, V.A.; Kumar, N.; Panicker, D.; Jin, J.; Chua, N.H.; Sarojam, R.
    Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis (2020), J. Exp. Bot., 71, 3638-3652 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.318 gene EGS1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3). Transient coexpression of ObCAAT1 and/o ObCAAT2 with ObEGS1 under control of CMV 35S promoter in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection, the transgenic leaves produce eugenol only when external coniferyl alcohol is added Ocimum basilicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+ Ocimum basilicum
-
 eugenol + acetate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Ocimum basilicum Q15GI4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.318 recombinant GST-tagged enzyme EGS1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.318 leaf in leaves, not in leaf stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information no expression of EGS1 in roots Ocimum basilicum
-
1.1.1.318 peltate glandular trichome PGTs Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum basilicum eugenol + acetate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.318 ? x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Ocimum basilicum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.318 EGS1
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 1
-
 Ocimum basilicum
1.1.1.318 ObEGS1
-
 Ocimum basilicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.318 22
-
 assay at room temperature Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.318 6
-
 assay at Ocimum basilicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.318 NADP+
-
 Ocimum basilicum
1.1.1.318 NADPH
-
 Ocimum basilicum

General Information

EC Number General Information Comment Organism
1.1.1.318 metabolism PGT-enriched BAHD acyltransferases, encoded by genes ObCAAT1 and ObCAAT2, convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis, followed by reduction of coniferyl acetate to eugenol and acetate catalyzed by eugenol synthase EGS1. Both ObCAAT1 and ObCAAT2 in combination with ObEGS1 produce eugenol The phenylpropene biosynthesis is not coupled to lignification in sweet basil. Plant phenylpropanoid pathway, overview Ocimum basilicum
1.1.1.318 additional information ObCAAT1-RNAi transgenic lines show decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives Ocimum basilicum
1.1.1.318 physiological function sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase Ocimum basilicum