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Literature summary extracted from

  • Kumar, A.; Subramanian Manimekalai, M.S.; Grueber, G.
    Substrate-induced structural alterations of Mycobacterial mycothione reductase and critical residues involved (2018), FEBS Lett., 592, 568-585 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.15 expressed in Escherichia coli DH5alpha cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.15 D158A/Y159A the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme Mycobacterium tuberculosis
1.8.1.15 S144A the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme Mycobacterium tuberculosis
1.8.1.15 S268A the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.15 95000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.15 mycothione + NADPH + H+ Mycobacterium tuberculosis
-
2 mycothiol + NADP+
-
?
1.8.1.15 mycothione + NADPH + H+ Mycobacterium tuberculosis H37Rv
-
2 mycothiol + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.15 Mycobacterium tuberculosis
-
-
-
1.8.1.15 Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.15 mycothione + NADPH + H+
-
Mycobacterium tuberculosis 2 mycothiol + NADP+
-
?
1.8.1.15 mycothione + NADPH + H+
-
Mycobacterium tuberculosis H37Rv 2 mycothiol + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.15 homodimer 2 * 49800, apoenzyme in solution, calculated from amino acid sequence Mycobacterium tuberculosis
1.8.1.15 homotetramer 4 * 49800, enzyme bound to NADPH, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.8.1.15 MTR
-
Mycobacterium tuberculosis
1.8.1.15 NADPH-dependent mycothione reductase
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.15 FAD
-
Mycobacterium tuberculosis
1.8.1.15 NADPH dependent on Mycobacterium tuberculosis