Any feedback?
Literature summary extracted from
- Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production (1986), J. Bacteriol., 167, 863-869.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.28 | expression in Escherichia coli | Zymomonas mobilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.28 | ethanol | 16% inhibition at 2 M, 48% inhibition at 4.7 M | Zymomonas mobilis |  |
| 1.1.99.28 | sorbitol | 0.8 M, 27% inhibition | Zymomonas mobilis | |
| 3.1.1.17 | ethanol | 10 mM, 20% inhibition | Zymomonas mobilis | |
| 3.1.1.17 | metal-complexing agents | activity is restored by excess of Ca2+, Mn2+ or Zn2+ | Zymomonas mobilis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.28 | 40000 | - |
4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
| 1.1.99.28 | 170000 | - |
gel filtration at pH 5.0 | Zymomonas mobilis |
| 1.1.99.28 | 300000 | - |
gel filtration at pH 7.0 | Zymomonas mobilis |
| 3.1.1.17 | 30000 | - |
3 * 30000 or 4 * 44000, SDS-PAGE | Zymomonas mobilis |
| 3.1.1.17 | 44000 | - |
3 * 30000 or 4 * 44000, SDS-PAGE | Zymomonas mobilis |
| 3.1.1.17 | 90000 | - |
gel filtration | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.28 | D-Glucose + D-fructose | Zymomonas mobilis | enzyme is responsible for sorbitol production | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.28 | Zymomonas mobilis | - |
- |
- |
| 3.1.1.17 | Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.28 | - |
Zymomonas mobilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.28 | 200 | - |
- |
Zymomonas mobilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.28 | 2-Deoxy-D-glucose + D-fructose | at 6% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Galactose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-glucose + D-fructose | - |
Zymomonas mobilis | D-glucono-1,5-lactone + D-sorbitol | - |
? | |
| 1.1.99.28 | D-Glucose + D-fructose | enzyme is responsible for sorbitol production | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Glucose + D-xylulose | at 7% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Glucose + dihydroxyacetone | at 6% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Glucose + L-sorbose | at 0.5% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Mannose + D-fructose | at 12% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | D-Xylose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | DL-Glyceraldehyde + D-fructose | at 1.5% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 1.1.99.28 | L-Arabinose + D-fructose | at 3% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| 3.1.1.17 | D-glucono-1,5-lactone + H2O | gluconolactone | Zymomonas mobilis | D-gluconate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.28 | tetramer | 4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
| 3.1.1.17 | ? | 3 * 30000 or 4 * 44000, SDS-PAGE | Zymomonas mobilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.28 | 0.8 | - |
sorbitol | - |
Zymomonas mobilis | |
| 1.1.99.28 | 0.8 | - |
gluconolactone | - |
Zymomonas mobilis | |
| 1.1.99.28 | 200 | - |
D-glucose | - |
Zymomonas mobilis | |
| 1.1.99.28 | 200 | - |
D-fructose | - |
Zymomonas mobilis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.28 | 6.2 | - |
- |
Zymomonas mobilis |
| 3.1.1.17 | 7 | - |
- |
Zymomonas mobilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.28 | 5 | 7.1 | pH 5.0: about 35% of maximal activity, pH 7.1: about 30% of maximal activity | Zymomonas mobilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.28 | NADP+ | tightly bound as hydrogen carrier | Zymomonas mobilis | |
| 1.1.99.28 | NADPH | tightly bound as hydrogen carrier | Zymomonas mobilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
