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Literature summary extracted from

  • Fang, L.; King, J.
    Purification and characterization of folylpolyglutamate synthetase (FPGS) from cultured cells of Datura innoxia (1997), J. Plant Physiol., 151, 528-534.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 6.8
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Datura inoxia
6.3.2.17 35.7
-
 ATP
-
 Datura inoxia
6.3.2.17 150
-
 L-Glu
-
 Datura inoxia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 Mg2+ required Datura inoxia
6.3.2.17 Mg2+ maximal activity at 20 mM Datura inoxia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 68000
-
 gel filtration Datura inoxia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.17 additional information Datura inoxia enzyme of folate biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Datura inoxia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17
-
 Datura inoxia

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 stable at 0-4°C or at -20°C Datura inoxia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Datura inoxia ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Datura inoxia ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Datura inoxia ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Datura inoxia ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Datura inoxia ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
6.3.2.17 ATP + methotrexate + Glu
-
 Datura inoxia ADP + phosphate + methotrexyl-Glu
-
 ?
6.3.2.17 additional information enzyme of folate biosynthetic pathway Datura inoxia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 monomer 1 * 68000, SDS-PAGE Datura inoxia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.5
-
-
 Datura inoxia