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Literature summary extracted from

  • Davis, J.S.; Balinsky, J.B.; Harington, J.S.; Shepherd, J.B.
    Assay, purification, properties and mechanism of action of gamma-glutamylcysteine synthetase from the liver of the rat and Xenopus laevis (1973), Biochem. J., 133, 667-678.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.2 Gly stimulates Rattus norvegicus
6.3.2.2 Gly no effect Xenopus laevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 glutathione inhibited by both GSSG and GSH Rattus norvegicus
6.3.2.2 glutathione GSSG inhibits, GSH has no inhibitory effect Xenopus laevis
6.3.2.2 K+
-
 Rattus norvegicus
6.3.2.2 K+
-
 Xenopus laevis
6.3.2.2 NAD+
-
 Rattus norvegicus
6.3.2.2 NAD+
-
 Xenopus laevis
6.3.2.2 NADH
-
 Rattus norvegicus
6.3.2.2 NADH
-
 Xenopus laevis
6.3.2.2 NADP+
-
 Rattus norvegicus
6.3.2.2 NADP+
-
 Xenopus laevis
6.3.2.2 NADPH
-
 Rattus norvegicus
6.3.2.2 NADPH
-
 Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-
6.3.2.2 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2
-
 Rattus norvegicus
6.3.2.2
-
 Xenopus laevis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.2 ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine bi bi uni uni ping pong mechanism, with glutamyl-enzyme as intermediate before the addition of cys and the release of gamma-glutamylcysteine Xenopus laevis
a
6.3.2.2 ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine tri tri sequential mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 liver
-
 Rattus norvegicus
-
6.3.2.2 liver
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 additional information
-
-
 Rattus norvegicus
6.3.2.2 additional information
-
-
 Xenopus laevis

Storage Stability

EC Number Storage Stability Organism
6.3.2.2 4°C, or in the frozen state, 25-30% loss of activity Xenopus laevis
6.3.2.2 4°C, or in the frozen state, stable for 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Xenopus laevis ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 7.7
-
-
 Rattus norvegicus
6.3.2.2 7.7
-
-
 Xenopus laevis