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Literature summary extracted from

  • Dvorakova, J.
    Phytase: source, preparation and exploitation (1998), Folia Microbiol. (Praha), 43, 323-338.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 nutrition addition of Aspergillus niger phytase to the flour containing wheat bran increases iron absorption in humans Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.04
-
myo-inositol hexakisphosphate
-
Bacillus subtilis
3.1.3.8 0.04
-
myo-inositol hexakisphosphate
-
Schwanniomyces castellii
3.1.3.8 0.04
-
myo-inositol hexakisphosphate strain NRRL 3135 Aspergillus niger
3.1.3.8 0.13
-
myo-inositol hexakisphosphate
-
Escherichia coli
3.1.3.8 0.15
-
myo-inositol hexakisphosphate
-
Rhizopus microsporus var. oligosporus
3.1.3.8 0.25
-
myo-inositol hexakisphosphate
-
Citrobacter freundii
3.1.3.8 0.3
-
myo-inositol hexakisphosphate
-
Raoultella terrigena
3.1.3.8 0.35
-
myo-inositol hexakisphosphate
-
Aspergillus carbonarius
3.1.3.8 0.39
-
myo-inositol hexakisphosphate
-
Aspergillus niger
3.1.3.8 0.44
-
myo-inositol hexakisphosphate strain 92 Aspergillus niger
3.1.3.8 0.48
-
myo-inositol hexakisphosphate strain IIIAn/8 Aspergillus niger
3.1.3.8 0.5
-
myo-inositol hexakisphosphate Bacillus subtilis var. natto Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
Bacillus subtilis
-
-
3.1.3.8 extracellular
-
Aspergillus niger
-
-
3.1.3.8 extracellular
-
Candida tropicalis
-
-
3.1.3.8 extracellular
-
Kluyveromyces marxianus
-
-
3.1.3.8 extracellular
-
Torulopsis candida
-
-
3.1.3.8 extracellular
-
Neurospora sp.
-
-
3.1.3.8 extracellular
-
Aspergillus oryzae
-
-
3.1.3.8 extracellular
-
Aspergillus terreus
-
-
3.1.3.8 extracellular
-
Aspergillus sp.
-
-
3.1.3.8 extracellular
-
Penicillium sp.
-
-
3.1.3.8 extracellular
-
Aspergillus fumigatus
-
-
3.1.3.8 extracellular
-
Penicillium camemberti
-
-
3.1.3.8 extracellular
-
Mucor sp.
-
-
3.1.3.8 extracellular
-
Aspergillus carneus
-
-
3.1.3.8 extracellular
-
Rhizopus microsporus var. oligosporus
-
-
3.1.3.8 extracellular
-
Debaryomyces castellii
-
-
3.1.3.8 intracellular
-
Escherichia coli 5622
-
3.1.3.8 intracellular
-
Citrobacter freundii 5622
-
3.1.3.8 intracellular
-
Raoultella terrigena 5622
-
3.1.3.8 intracellular
-
Klebsiella sp. 5622
-
3.1.3.8 intracellular
-
Klebsiella oxytoca 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 36000 38000 Bacillus subtilis var. natto Bacillus subtilis
3.1.3.8 36500
-
-
Bacillus subtilis
3.1.3.8 40000
-
-
Raoultella terrigena
3.1.3.8 42000
-
-
Escherichia coli
3.1.3.8 60000 81000
-
Penicillium camemberti
3.1.3.8 80000
-
-
Aspergillus niger
3.1.3.8 85000 100000 strain NRRL3135 Aspergillus niger
3.1.3.8 100000
-
strain 92 Aspergillus niger
3.1.3.8 200000
-
-
Aspergillus niger
3.1.3.8 214000
-
-
Aspergillus terreus
3.1.3.8 490000
-
-
Klebsiella oxytoca
3.1.3.8 700000
-
high molecular weight form, gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus carbonarius
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Aspergillus carneus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Aspergillus fumigatus
-
-
-
3.1.3.8 Aspergillus niger
-
-
-
3.1.3.8 Aspergillus niger 92
-
-
-
3.1.3.8 Aspergillus niger IIIAn/8
-
-
-
3.1.3.8 Aspergillus niger NRRL 3135
-
-
-
3.1.3.8 Aspergillus oryzae
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Aspergillus sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Aspergillus terreus
-
-
-
3.1.3.8 Bacillus subtilis
-
var. natto
-
3.1.3.8 Candida tropicalis
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Citrobacter freundii
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Debaryomyces castellii
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Escherichia coli
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Klebsiella aerogenes
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Klebsiella oxytoca
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Klebsiella oxytoca
-
MO-3
-
3.1.3.8 Klebsiella oxytoca MO-3
-
MO-3
-
3.1.3.8 Klebsiella sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Kluyveromyces marxianus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Mucor sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Neurospora sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Paramecium sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Penicillium camemberti
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Penicillium sp.
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Raoultella terrigena
-
-
-
3.1.3.8 Rhizopus microsporus var. oligosporus
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Saccharomyces cerevisiae
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Schwanniomyces castellii
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Torulopsis candida
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Bacillus subtilis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Escherichia coli 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Saccharomyces cerevisiae 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Klebsiella aerogenes 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Candida tropicalis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Kluyveromyces marxianus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Torulopsis candida 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Citrobacter freundii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Neurospora sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus oryzae 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus terreus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Raoultella terrigena 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Penicillium sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Penicillium camemberti 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus carbonarius 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Klebsiella oxytoca 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Mucor sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus carneus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Rhizopus microsporus var. oligosporus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Debaryomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Schwanniomyces castellii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Paramecium sp. 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger 92 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger NRRL 3135 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus niger IIIAn/8 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Klebsiella oxytoca MO-3 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
-
-
Aspergillus carneus
3.1.3.8 45
-
-
Penicillium camemberti
3.1.3.8 50
-
-
Aspergillus oryzae
3.1.3.8 52
-
-
Citrobacter freundii
3.1.3.8 53
-
-
Aspergillus carbonarius
3.1.3.8 55
-
-
Bacillus subtilis
3.1.3.8 55
-
-
Escherichia coli
3.1.3.8 55
-
-
Aspergillus niger
3.1.3.8 55
-
-
Klebsiella oxytoca
3.1.3.8 55
-
-
Rhizopus microsporus var. oligosporus
3.1.3.8 58
-
-
Aspergillus niger
3.1.3.8 58
-
-
Raoultella terrigena
3.1.3.8 60
-
-
Neurospora sp.
3.1.3.8 60
-
-
Aspergillus fumigatus
3.1.3.8 60
-
var. natto Bacillus subtilis
3.1.3.8 70
-
-
Aspergillus terreus
3.1.3.8 77
-
-
Klebsiella oxytoca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2.7
-
and a second optimum at pH 5.0 Citrobacter freundii
3.1.3.8 3
-
-
Aspergillus niger
3.1.3.8 3
-
-
Penicillium camemberti
3.1.3.8 4.4
-
-
Schwanniomyces castellii
3.1.3.8 4.5
-
-
Escherichia coli
3.1.3.8 4.5
-
-
Klebsiella aerogenes
3.1.3.8 4.5
-
-
Aspergillus terreus
3.1.3.8 4.5
-
-
Rhizopus microsporus var. oligosporus
3.1.3.8 4.7
-
-
Aspergillus carbonarius
3.1.3.8 5
-
-
Aspergillus niger
3.1.3.8 5
-
-
Raoultella terrigena
3.1.3.8 5
-
-
Aspergillus fumigatus
3.1.3.8 5
-
and a second optimum which is lower at pH 2.7 Citrobacter freundii
3.1.3.8 5 6
-
Neurospora sp.
3.1.3.8 5 6
-
Klebsiella oxytoca
3.1.3.8 5.5
-
-
Aspergillus niger
3.1.3.8 5.5
-
-
Aspergillus oryzae
3.1.3.8 5.5
-
-
Aspergillus terreus
3.1.3.8 5.6
-
-
Aspergillus carneus
3.1.3.8 5.6
-
-
Rhizopus microsporus var. oligosporus
3.1.3.8 6 6.5
-
Aspergillus fumigatus
3.1.3.8 6 6.5 var. natto Bacillus subtilis
3.1.3.8 7 7.5
-
Bacillus subtilis