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Literature summary extracted from

  • Macnicol, P.K.
    Homoglutathione and glutathione synthetases of legume seedlings: partial purification and substrate specificity (1987), Plant Sci., 53, 229-235.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 adenosine 5'-phosphosulfate slight Pisum sativum
6.3.2.3 ADP strong Pisum sativum
6.3.2.3 AMP slight Pisum sativum
6.3.2.23 adenosine 5'-phosphosulfate 5 mM: 10-30% inhibition Vigna radiata var. radiata
6.3.2.23 ADP 5 mM: 70% inhibition Vigna radiata var. radiata
6.3.2.23 AMP 5 mM: 10-30% inhibition Vigna radiata var. radiata
6.3.2.23 cysteine 5 mM: 15% inhibition Vigna radiata var. radiata
6.3.2.23 glutathione 5 mM: 15% inhibition Vigna radiata var. radiata
6.3.2.23 additional information methionine, homocysteine, S-adenosyl-methionine, serine, all 5 mM, are no inhibitors Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.19
-
 Gly
-
 Pisum sativum
6.3.2.3 0.22
-
 gamma-Glu-2-aminobutyrate
-
 Pisum sativum
6.3.2.3 0.25
-
 ATP
-
 Pisum sativum
6.3.2.3 0.28
-
 gamma-Glu-S-methylcysteine
-
 Pisum sativum
6.3.2.3 0.35
-
 gamma-Glu-Cys
-
 Pisum sativum
6.3.2.3 14
-
 beta-Ala
-
 Pisum sativum
6.3.2.23 0.33
-
 beta-Alanine
-
 Vigna radiata var. radiata
6.3.2.23 0.45
-
 ATP
-
 Vigna radiata var. radiata
6.3.2.23 0.75
-
 gamma-glutamylcysteine
-
 Vigna radiata var. radiata
6.3.2.23 7.5
-
 glycine
-
 Vigna radiata var. radiata
6.3.2.23 8.3
-
 gamma-aminobutyrate
-
 Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.23 Mg2+ requirement Vigna radiata var. radiata
6.3.2.23 Mg2+ 2-3 mM optimal Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.3 85000
-
 gel filtration Pisum sativum
6.3.2.23 85000
-
 gel filtration Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Vigna radiata var. radiata
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homoglutathione ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Pisum sativum
-
-
-
6.3.2.23 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3
-
 Pisum sativum
6.3.2.23 partial Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.3 seedling light-grown Pisum sativum
-
6.3.2.23 aerial part
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 0.033
-
-
 Pisum sativum
6.3.2.23 0.042
-
 gamma-glutamylcysteine + beta-alanine Vigna radiata var. radiata

Storage Stability

EC Number Storage Stability Organism
6.3.2.23 -20°C, more than 1 mg enzyme/ml in potassium phosphate buffer, 30-50% glycerol, 1 mM dithiothreitol, stable for at least 4 months Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-aminobutyrate + Gly
-
 Pisum sativum ADP + phosphate + ophthalmic acid
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Ala beta-alanine Pisum sativum ADP + phosphate + gamma-Glu-Cys-Ala
-
 ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Pisum sativum ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-Glu-S-methylcysteine + Gly
-
 Pisum sativum ADP + phosphate + gamma-Glu-S-methylcysteinyl-Gly
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + beta-Ala
-
 Pisum sativum ADP + phosphate + gamma-Glu-L-Cys-beta-Ala
-
 ?
6.3.2.23 ATP + gamma-glutamylcysteine + gamma-aminobutyrate
-
 Vigna radiata var. radiata ADP + phosphate + gamma-glutamylcysteinyl-4-aminobutyrate
-
 ?
6.3.2.23 ATP + gamma-glutamylcysteine + glycine
-
 Vigna radiata var. radiata ADP + phosphate + gamma-glutamylcysteinylglycine
-
 ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Vigna radiata var. radiata ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homoglutathione ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.23 25
-
 assay at Vigna radiata var. radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.23 40
-
 20 h, 50 mM potassium phosphate buffer, pH 7.5: 15% remaining activity. 20 h, buffer plus 10% (v/v) glycerol: 30% remaining activity. 20 h, buffer with glycerol plus 1 mM dithiothreitol: 50% remaining activity. 20 h, buffer with glycerol and dithiothreitol plus 10 mg/ml bovine serum albumin: 85% remaining activity Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8.4
-
-
 Pisum sativum
6.3.2.23 8.4
-
-
 Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.23 7 10 50% of maximal activity at pH 7 and pH 10 Vigna radiata var. radiata