EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.3 | mutants Cys122Ala, Cys195Ala, Cys222Ala and Cys289Ala show no critical loss of activity. Multiple replacement of Cys residues, however, decreases enzymatic activity to 45-26% of the activity of the wild-type enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.3 | 5,5'-dithiobis(2-nitrobenzoate) | Cys289 is the only amino acid residue reactive with DTNB. Modification of Cys289 with DTNB results in complete loss of the catalytic activity | Escherichia coli | |
6.3.2.3 | NEM | - |
Escherichia coli | |
6.3.2.3 | PCMB | - |
Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.3 | Escherichia coli | - |
wild-type and mutant enzymes with Cys residues replaced by Ala | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.3 | ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli | ADP + phosphate + glutathione | - |
? |