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Literature summary extracted from

  • Lehmann, M.; Tshisuaka, B.; Fetzner, S.; Roger, P.; Lingens, F.
    Purification and characterization of isoquinoline 1-oxidoreductase from Pseudomonas diminuata 7, a novel molybdenum-containing hydroxylase (1994), J. Biol. Chem., 269, 11254-11260.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.16 1,10-phenanthroline
-
 Brevundimonas diminuta
1.3.99.16 5-Hydroxyisoquinoline rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide Brevundimonas diminuta
1.3.99.16 arsenite
-
 Brevundimonas diminuta
1.3.99.16 CN-
-
 Brevundimonas diminuta
1.3.99.16 Isoquinoline slow inhibition in absence of an electron acceptor Brevundimonas diminuta
1.3.99.16 methanol
-
 Brevundimonas diminuta
1.3.99.16 p-hydroxymercuribenzoate
-
 Brevundimonas diminuta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.16 Iron enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters Brevundimonas diminuta
1.3.99.16 Mo enzyme contains 0.85 g-atom of molybdenum per mol of enzyme Brevundimonas diminuta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.16 16000
-
 1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta
1.3.99.16 80000
-
 1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta
1.3.99.16 93000 95000 gel filtration, analytical ultracentrifugation Brevundimonas diminuta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.16 Isoquinoline + acceptor + H2O Brevundimonas diminuta first enzyme of the isoquinoline catabolism ?
-
 ?
1.3.99.16 Isoquinoline + acceptor + H2O Brevundimonas diminuta 7 first enzyme of the isoquinoline catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.16 Brevundimonas diminuta
-
-
-
1.3.99.16 Brevundimonas diminuta 7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.16
-
 Brevundimonas diminuta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.16 13.87
-
-
 Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.16 5-hydroxyisoquinoline + acceptor + H2O
-
 Brevundimonas diminuta 1,5-dihydroisoquinoline + reduced acceptor
-
 ?
1.3.99.16 5-hydroxyisoquinoline + acceptor + H2O
-
 Brevundimonas diminuta 7 1,5-dihydroisoquinoline + reduced acceptor
-
 ?
1.3.99.16 isoquinoline + acceptor + H2O acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone Brevundimonas diminuta 1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
 ?
1.3.99.16 isoquinoline + acceptor + H2O acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone Brevundimonas diminuta 7 1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
 ?
1.3.99.16 Isoquinoline + acceptor + H2O first enzyme of the isoquinoline catabolism Brevundimonas diminuta ?
-
 ?
1.3.99.16 Isoquinoline + acceptor + H2O first enzyme of the isoquinoline catabolism Brevundimonas diminuta 7 ?
-
 ?
1.3.99.16 phthalazine + acceptor + H2O at 89% of the activity compared to isoquinoline Brevundimonas diminuta 1-oxophthalazine + reduced acceptor
-
 ?
1.3.99.16 phthalazine + acceptor + H2O at 89% of the activity compared to isoquinoline Brevundimonas diminuta 7 1-oxophthalazine + reduced acceptor
-
 ?
1.3.99.16 quinazoline + acceptor + H2O at 45% of the activity compared to isoquinoline Brevundimonas diminuta 4-hydroxyquinazoline + reduced acceptor
-
 ?
1.3.99.16 quinazoline + acceptor + H2O at 45% of the activity compared to isoquinoline Brevundimonas diminuta 7 4-hydroxyquinazoline + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.16 dimer 1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.16 55 60
-
 Brevundimonas diminuta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.99.16 60
-
 5 min, rapid decrease of activity above Brevundimonas diminuta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.16 8.5
-
-
 Brevundimonas diminuta

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.16 additional information enzyme contains no FAD Brevundimonas diminuta
1.3.99.16 Pterin molybdenum cofactor
-
 Brevundimonas diminuta