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Literature summary extracted from

  • Daubner, S.C.; Schrimsher, J.L.; Schendel, F.J.; Young, M.; Henikoff, S.; Patterson, D.; Stubbe, J.; Benkovic, S.J.
    A multifunctional protein possessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase activities in de novo purine biosynthesis (1985), Biochemistry, 24, 7059-7062.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2
-
 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 110000
-
 SDS-PAGE Gallus gallus
6.3.4.13 additional information
-
 MW 49000, GAR domain of the trifunctional enzyme, gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Saccharomyces cerevisiae
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Schizosaccharomyces pombe
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Drosophila melanogaster essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
6.3.4.13 ATP + 5-phosphoribosylamine + Gly Gallus gallus enzyme of purine biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Drosophila melanogaster
-
 fruit fly
-
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Saccharomyces cerevisiae
-
 yeast
-
2.1.2.2 Schizosaccharomyces pombe
-
 fission yeast
-
6.3.3.1 Gallus gallus
-
 trifunctional enzyme with AIR synthetase activity, phosphoribosylglycinamide synthetase activity, and phosphoribosylglycinamide transformylase activity
-
6.3.4.13 Gallus gallus
-
 trifunctional enzyme with phosphoribosylamine-glycine ligase activity, glycinamide ribonucleotide transformylase activity, and aminoimidazole ribonucleotide synthetase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Gallus gallus
6.3.3.1 trifunctional enzyme with AIR synthetase activity, phosphoribosylglycinamide synthetase activity, and phosphoribosylglycinamide transformylase activity Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 liver
-
 Gallus gallus
-
6.3.3.1 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 0.95
-
-
 Gallus gallus
6.3.3.1 0.32
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Drosophila melanogaster tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Saccharomyces cerevisiae tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Schizosaccharomyces pombe tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Drosophila melanogaster tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase Gallus gallus ?
-
 ?
6.3.3.1 ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine
-
 Gallus gallus ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole
-
 ?
6.3.4.13 ATP + 5-phosphoribosylamine + Gly
-
 Gallus gallus ADP + phosphate + 5-phosphoribosylglycinamide
-
 ?
6.3.4.13 ATP + 5-phosphoribosylamine + Gly enzyme of purine biosynthetic pathway Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.13 ? GAR domain of the multifunctional enzyme has a MW of 49000, SDS-PAGE, the multifunctional enzyme has a MW of 110000, SDS-PAGE Gallus gallus