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Literature summary extracted from

  • Natarajan, R.; Ghosh, S.; McLean Grogan, W.
    Catalytic properties of the purified rat hepatic cytosolic cholesteryl ester hydrolase (1996), Biochem. Biophys. Res. Commun., 225, 413-419.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 sodium taurocholic acid 0.25 mM prevents aggregation of the enzyme into an inactive state, 3fold stimulation with more lipophilic substrates at 5 mM Rattus sp.

General Stability

EC Number General Stability Organism
3.1.1.13 mild digestion results in progressive and selective loss of activity Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 Cu2+
-
 Rattus sp.
3.1.1.13 Hg2+
-
 Rattus sp.
3.1.1.13 iodoacetamide modest inactivation Rattus sp.
3.1.1.13 N-ethylmaleimide modest inactivation Rattus sp.
3.1.1.13 p-mercuribenzoate 30% inhibition at 3 mM Rattus sp.
3.1.1.13 Phenylmethylsulfonylfluoride 50% inhibition at 0.1 mM Rattus sp.
3.1.1.13 sodium taurocholic acid inhibition higher than 2.5 mM for cholesteryl oleate and triolein hydrolysis Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 cytosol
-
 Rattus sp. 5829
-
3.1.1.13 microsome
-
 Rattus sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Rattus sp.
-
 cholesterol + fatty acid
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Rattus sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 liver
-
 Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O
-
 Rattus sp. cholesterol + fatty acid
-
 r
3.1.1.13 cholesteryl linoleate + H2O activity lower than with oleate esters Rattus sp. cholesterol + linoleic acid
-
 r
3.1.1.13 cholesteryl oleate + H2O
-
 Rattus sp. cholesterol + oleic acid
-
 r
3.1.1.13 p-nitrophenyl acetate + H2O low activity Rattus sp. p-nitrophenol + acetic acid
-
 r
3.1.1.13 p-nitrophenyl caprylate + H2O activity increases with more lipophilic substrates Rattus sp. p-nitrophenol + caprylic acid
-
 ?
3.1.1.13 triolein + H2O
-
 Rattus sp. dioleoylglycerol + oleic acid
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 CEH
-
 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.13 0.1
-
 Phenylmethylsulfonylfluoride
-
 Rattus sp.