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Literature summary extracted from
- Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties (1977), J. Biochem., 81, 1209-1215.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | adekatol | 4fold activation at 0.01% w/v | Fusarium oxysporum | |
| 3.1.1.13 | cholic acid | slight activation | Fusarium oxysporum |  |
| 3.1.1.13 | ethyl ether | slight activation at 1-3% v/v | Fusarium oxysporum | |
| 3.1.1.13 | sodium deoxycholate | slight activation | Fusarium oxysporum | |
| 3.1.1.13 | Triton X-100 | 5fold activation at 0.01% w/v | Fusarium oxysporum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | lecithin | - |
Fusarium oxysporum | |
| 3.1.1.13 | lysolecithin | - |
Fusarium oxysporum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | Fusarium oxysporum | - |
cholesterol + fatty acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | Fusarium oxysporum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.13 | partial, 270fold by CaCl2-precipitation, Sephadex G-75 chromatography | Fusarium oxysporum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | cell culture | - |
Fusarium oxysporum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 9.01 | - |
- |
Fusarium oxysporum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl acetate + H2O | - |
Fusarium oxysporum | cholesterol + acetic acid | - |
? | |
| 3.1.1.13 | cholesteryl butyrate + H2O | - |
Fusarium oxysporum | cholesterol + butanoic acid | - |
? | |
| 3.1.1.13 | cholesteryl esters + H2O | - |
Fusarium oxysporum | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl linoleate + H2O | most suitable substrate | Fusarium oxysporum | cholesterol + linoleic acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Fusarium oxysporum | cholesterol + oleic acid | - |
? | |
| 3.1.1.13 | cholesteryl palmitate + H2O | low activity | Fusarium oxysporum | cholesterol + palmitic acid | - |
? | |
| 3.1.1.13 | cholesteryl valerate + H2O | - |
Fusarium oxysporum | cholesterol + valeric acid | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 50 | - |
10% loss of activity after 90 min | Fusarium oxysporum |
| 3.1.1.13 | 60 | - |
complete loss of activity | Fusarium oxysporum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 7 | - |
- |
Fusarium oxysporum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 4 | 10 | stable for 20 h at 25°C | Fusarium oxysporum |
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