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Literature summary extracted from

  • Chen, D.; Dennis, E.A.
    Expression and characterization of human group V phospholipase A2 (1998), Biochim. Biophys. Acta, 1394, 57-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into pET28a(+) vector, transformation of Escherichia coli strain BL21 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 3-(3-acetamido-2-benzyl-2-ethylindolyl-5-oxy)propane phosphonic acid LY311727, IC50 value of 36 nM, group IIa and V sPLA2 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 secretory granule
-
 Homo sapiens 30141
-
3.1.1.4 secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Mus musculus
3.1.1.4 Ca2+
-
 Homo sapiens
3.1.1.4 Ca2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 16000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens influence on platelet aggregation ?
-
 ?
3.1.1.4 phospholipids + H2O Rattus norvegicus influence on platelet aggregation ?
-
 ?
3.1.1.4 phospholipids + H2O Homo sapiens signal transduction ?
-
 ?
3.1.1.4 phospholipids + H2O Mus musculus eicosanoids ?
-
 ?
3.1.1.4 phospholipids + H2O Homo sapiens eicosanoids ?
-
 ?
3.1.1.4 phospholipids + H2O Rattus norvegicus eicosanoids ?
-
 ?
3.1.1.4 phospholipids + H2O Homo sapiens inflammatory diseases ?
-
 ?
3.1.1.4 phospholipids + H2O Rattus norvegicus inflammatory diseases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-
3.1.1.4 Mus musculus
-
-
-
3.1.1.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 Ni-NTA affinity chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 heart
-
 Homo sapiens
-
3.1.1.4 leukocyte peripheral blood Homo sapiens
-
3.1.1.4 liver
-
 Homo sapiens
-
3.1.1.4 liver
-
 Rattus norvegicus
-
3.1.1.4 lung
-
 Homo sapiens
-
3.1.1.4 lung
-
 Rattus norvegicus
-
3.1.1.4 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.5
-
 substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine Homo sapiens
3.1.1.4 1.8
-
 substrate: 1-palmitoyl-2-linoleoylphosphatidylcholine Homo sapiens
3.1.1.4 3.22
-
 substrate: 1,2-dipalmitoyl-phosphatidylcholine Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -4°C, for months without losing enzymatic activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1,2-dipalmitoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylcholine + linolic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylcholine + H2O group V sPLA2 Homo sapiens 1-palmitoyl-glycerophosphorylcholine + linolic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Rattus norvegicus ?
-
 ?
3.1.1.4 phospholipids + H2O signal transduction Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O eicosanoids Mus musculus ?
-
 ?
3.1.1.4 phospholipids + H2O eicosanoids Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O eicosanoids Rattus norvegicus ?
-
 ?
3.1.1.4 phospholipids + H2O inflammatory diseases Homo sapiens ?
-
 ?
3.1.1.4 phospholipids + H2O inflammatory diseases Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8.5
-
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.4 0.000036
-
 LY311727, IC50 value of 36 nM, group IIa and V sPLA2 Homo sapiens 3-(3-acetamido-2-benzyl-2-ethylindolyl-5-oxy)propane phosphonic acid