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Literature summary extracted from
- Cytosolic phospholipase A2 from human monocytic cells: characterization of substrate specificity and Ca2+-dependent membrane association (1993), Biochem. J., 293, 255-261.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | phosphate | phosphorylation needed for activity | Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | 2-mercaptoethanol | - |
Homo sapiens | |
| 3.1.1.4 | EDTA | - |
Homo sapiens | |
| 3.1.1.4 | EGTA | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.4 | cytoplasm | - |
Homo sapiens | 5737 | - |
| 3.1.1.4 | plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Ca2+ | Ca2+-dependent PLA2-association with cellular membranes at a concentration of 300-500 nM | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 78000 | - |
native PAGE | Homo sapiens |
| 3.1.1.4 | 100000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | phospholipids + H2O | Homo sapiens | eicosanoids | ? | - |
? | |
| 3.1.1.4 | phospholipids + H2O | Homo sapiens | inflammatory diseases | ? | - |
? | |
| 3.1.1.4 | phospholipids + H2O | Homo sapiens | release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | column chromatography, gel electrophoresis, 1206fold | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | leukocyte | peripheral blood | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 0.0609 | - |
substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.4 | -70°C, with bovine serum albumin, loss of enzyme activity only partially avoided | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O | - |
Homo sapiens | 1-acyl-sn-glycerophosphoethanolamine + arachidonic acid | - |
? | |
| 3.1.1.4 | 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O | - |
Homo sapiens | 1-palmitoyl-glycerophosphorylcholine + arachidonic acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + arachidonic acid | - |
Homo sapiens | 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine + carboxylic acid | - |
r | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Homo sapiens | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylcholine + linoleic acid | - |
Homo sapiens | lineoyl phosphatidylcholine | - |
? | |
| 3.1.1.4 | phosphatidylethanolamine + arachidonic acid | - |
Homo sapiens | arachidonoyl phosphatidylethanolamine | - |
r | |
| 3.1.1.4 | phosphatidylethanolamine + linoleic acid | - |
Homo sapiens | lineoyl phosphatidylethanolamine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylinositol + arachidonic acid | - |
Homo sapiens | arachidonoyl phosphatidylinositol + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylinositol + linoleic acid | - |
Homo sapiens | lineoyl phosphatidylinositol + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylserine + arachidonic acid | - |
Homo sapiens | arachidonoyl phosphatidylserine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylserine + linoleic acid | - |
Homo sapiens | lineoyl phosphatidylserine + fatty acid | - |
? | |
| 3.1.1.4 | phospholipids + H2O | eicosanoids | Homo sapiens | ? | - |
? | |
| 3.1.1.4 | phospholipids + H2O | inflammatory diseases | Homo sapiens | ? | - |
? | |
| 3.1.1.4 | phospholipids + H2O | release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | monomer | - |
Homo sapiens |
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